Scientific Frequently Asked Question

Results 1 - 100 of 150 for FAQ

FAQ	Category
Are all products available online? All catalog assays kits, assay consumables, assay development products, and inst...	Ordering
Are the Aß and Tau calibrators in the validated Human Aß42 and Human Total Tau kits native protein? The Total Tau assay is a recombinant Tau 441 protein expressed in E.coli. The A&...	Assays Ordering
Can I backup the WORKBENCH Software database? Yes, the Workbench software comes with a backup utility that can be executed in ...	Instrument Software
Can I cancel an order I placed online? Yes, but cancellations are handled individually depending on the production or s...	Ordering
Can I combine R-PLEX and U-PLEX with Prototype and/or T-PLEX Assays? Multiplexed U-PLEX and R-PLEX assays use Linkers to coat the plate with capture ...
Can I conjugate proteins and antibodies with SULFO-TAG in my lab? MSD provides SULFO-TAG NHS-Ester and you can follow the straightforward conjugat...	Assays
Can I denature the cell lysates? Yes, ionic detergents or urea can be used to denature proteins; however, these r...	Assays
Can I develop my own phosphoprotein assays? Yes, immobilizing antibodies on bare plates and labeling detection antibodies is...	Assays
Can I have my order shipped overnight? All MSD orders within the continental US are shipped overnight at no additional ...	Ordering
Can I increase my sample volume to improve the sensitivity of the assay? For the sample incubation step, most MSD assays use between 25-50 μL/well sam...	Assays
Can I move an MSD instrument myself? Should your instrument need to be relocated, we can assist with the relocation a...	Instrument
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Akt Kits? Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Ak...	Assays
Can I order additional calibrators and/or detection antibodies? Yes. Many calibrators and labeled secondary reagents are listed on our website u...	Reagents Diluents Buffers
Can I provide a quote number online with a order? Yes, you can reference your quote number in the Special Instructions box du...	Ordering
Can I perform R-PLEX Singleplex assays on plates other than Small Spot Streptavidin plates? R‑PLEX Singleplex assays can be developed on any MSD streptavidin- or avidin-coa...	Assays
Can I re-read a plate? No, once the plate has been read and the wells have been 'fired' through the ECL...	Instrument
Can I re-read a plate? No. Once the wells have been exposed to the electrochemiluminescence process, th...	Plates Instrument Software
Can I run my samples un-diluted to increase sensitivity? For optimal performance, you should use the minimum sample dilution recommended ...	Assays
Can I run tissue homogenates or other sample types with V-PLEX kits? MSD has not validated other sample types with the V-PLEX kits, but other sample ...	Assays
Can I save a shopping cart until my purchase order is ready? Yes, your shopping cart will be saved automatically if you sign out or leave the...	Ordering
Can I use 384-well plates with R-PLEX? You can use as little as 10 µL per well to coat a 384‑well plate. Calculat...	Assays
Can I use a different PC than the one supplied with the instrument? No. Each computer MSD provides has been configured to a particular instrument so...	Software Instrument
Can I use my own diluents and wash buffer when running R-PLEX assays? Diluents used to generate the reported data are recommended as starting diluents...	Assays
Can I use my own diluents and wash buffer when running R-PLEX assays? Diluents used to generate the reported data are recommended as starting diluents...	Assays
Can I use the same calibrator when testing sample types other than serum, plasma, or urine? Yes, the same calibrator and thus the same calibration curve can be used for mul...	Assays
Can I view my order status online? One of the benefits of placing all your orders online is the ability to see your...	Ordering
Can R-PLEX calibrators be purchased individually? The R-PLEX calibrators are only available as part of an R-PLEX Antibody Set....	Assays
Can sample dilutions other than the recommended dilutions be run? Can you lower the dilution factor to increase sensitivity? Dilution linearity for CSF is provided in the product insert and data sheets. To...	Assays
Can the Aß42 assay be run in a homogenous format? While the Aβ42 assay was validated with a washed assay, a homogenous protoc...	Assays
Can the assays be used with tissue lysates? Yes, brain tissue homogenates, liver homogenates, homogenates from Matrigel plug...	Assays
Can the IL-8 HA assay be purchased as a singleplex? No, the IL-8 HA is only available in select multiplex panels....	Assays
Can the Validated Human Aß42 and Human Total Tau assays be multiplexed together or with the other Aß peptide assays? The assays can be provided as a custom multiplex with comparable performance to ...	Assays
Can V-PLEX orders ship within the same day? No. V-PLEX orders generally ship within 1-3 business days....	Ordering Assays
Can we network the MSD instrument computer? If the system is under evaluation, it may not be networked. If the MSD system ha...	Instrument
Can we upgrade the Windows 2000 operating system to Windows XP? Please contact MSD Instrument Service Support before upgrading to XP. This can b...	Instrument Software
Can Windows updates be installed on the computer controlling the MSD instrument? Many of our customers have installed Windows® updates without problems, incl...	Instrument Software
Can you measure Aß42 levels in plasma with the validated Human Aß42 Kit? The assay was validated with human CSF. MSD is currently working on blood-based ...	Assays
I see a 'Client already has a session' error when booting up DISCOVERY WORKBENCH. This indicates that a duplicate of the DISCOVERY WORKBENCH software is running. ...	Instrument
Do I have to use the specific diluents provided on the R PLEX datasheet for my assay? What if I am multiplexing? The Diluent Compatibility table on the R-PLEX datasheet lists the diluents that ...	Assays
Does MSD offer a multiplex for Aß42, Total Tau, and p-Tau (181)? No, but the assays can be provided as a custom multiplex. However, the custom mu...	Assays
Does MSD offer the 12F4 antibody as a detection antibody with a 6E10 capture in the Human (6E10) Abeta 42 Ultra-Sensitive Kit? No. We currently do not offer 12F4 as a detection antibody. We have not found an...	Assays
Does MSD use controls in the development of validated assays? If so, are they samples or diluted standards? We test controls as part of the validation process for V‑PLEX assays. The t...	Assays
Does the Human Total Tau Kit work for mouse samples? We have tested mouse brain lysates with the Human Total Tau Kit and were unable ...	Assays
FAQs for V-PLEX Proinflammatory Panel 1 (mouse) Kits What is the difference between the 10-plex panel, a custom panel, and the s...	Assays
For R-PLEX assays, can I use less antibody to coat the plate? R-PLEX is provided as a matched antibody set. We do not recommend using less of ...
How are MSD assays validated? A cell model that exhibits regulation of a phosphoprotein of interest is selecte...
How are V-PLEX assays different from T-PLEX assays? Unlike T-PLEX assays (tissue culture, ultra-sensitive, whole cell lysate, serum/...	Assays
How are your new Phospho-Akt (Ser473) and Total Akt Kits different than your existing kits? Our new Phospho-Akt (Ser473) Kit (catalog# K150MND) and Total Akt Kit (catalog# ...
How can I control cross-reactivity in multiplex assays? MSD has several blockers available that can be added to lysis buffer or antibody...	Assays
How can I export each spot in 96- (384-) well format and do other data file customization? Click Run to activate the Run Options window, check the Export Format Custom box...	Instrument
How can I find my account manager's contact information? When you are online and signed in to our website, your account manager’s c...	Ordering
How can I obtain a Certificate of Analysis (COA)? COAs are available on our website. In order to locate the correct COA for any pr...	Assays
How can I place an order without registering online? Online ordering provides you with access to your customer specific pricing and e...	Ordering
How critical are the incubation times when running V-PLEX assays? Are there any steps where I can incubate the samples overnight? For consistent results, MSD recommends keeping incubation times consistent withi...	Assays
How did MSD decide which specific kidney biomarkers to target? MSD worked with the Critical Path Institute (C-Path) and the Predictive Safety T...	Assays
How do I add products to my shopping cart Find the product you are interested in by searching our site, browsing through o...	Ordering
How do I change my password? Sign in using your current password, then go to your Profile (see link at top of...	Ordering
How do I change my shipping or billing address? This can be accomplished by visiting your personal online Profile page. S...	Ordering
How do I delete my cart? Your online shopping cart can be deleted by: Navigating to the Cart Cl...	Ordering
How do I make changes to my Profile? As a registered user, you can update various portions of your online profile. Fi...	Ordering
How do I measure percent phosphorylation? Perform a lysate titration to establish the linear range of the assay. For sampl...	Assays
How do I read a partial plate? SECTOR instruments read plates in sectors. For example, the SECTOR Imager 6000 d...	Instrument Plates Software
How do I register for your site? Simply click the Register link at the top of any MSD webpage and follow the prom...	Ordering
How do I request special handling? Special shipping instructions can be included with each online order. When check...	Ordering
How do I see my customer-specific pricing? MSD customers can see their contract pricing online after registering for an onl...	Ordering
How do I sign in if I forgot my password? Go to the Sign In screen and click Forgot Password. On the next screen, enter th...	Ordering
How do I upgrade from an older version of DISCOVERY WORKBENCH? Instrument Service must upgrade the computer connected to your instrument, but y...	Software
How long should I leave the assay in read buffer before I read the plate? Unless otherwise directed in the protocol, incubation in read buffer is not reco...	Assays Reagents Diluents Buffers
How many samples can I run on a plate? MSD assays are provided in convenient 96- or 384-well microtiter plates. The num...	Assays Plates
How much secondary antibody do I use for my assay development? For initial assay development, it is recommended that a 1:1 primary:secondary an...	Assays
I am multiplexing R-PLEX with U-PLEX analytes. The R PLEX protocol says to dilute the U PLEX calibrators 10 fold into the calibrator blend. But the U PLEX certificate of analysis (COA) provides the calibrator concentrations for a 5-fold dilution. The U-PLEX Calibrators are reconstituted with a lower volume (high concentration...	Assays
I can't find my data file, where is it? Another user may have changed the Output Path in the Run window. Click Run to vi...	Instrument
I have controls that were made in CSF that do not recover between 80% and 120% (or the CVs are greater than 20%). Pooled CSF can have interferences that are not present in properly collected and...	Assays
I’m not getting any signal with my R-PLEX assay. Check to ensure that the reagents were added correctly. Make sure that you used ...	Assays
I measured the plate backwards, is my data correct? The SECTOR instruments have a sensor that tells the software the plate is backwa...	Instrument
I obtained low spike recovery for the osteopontin assay in the MSD Kidney Injury Panel 1 (rat) Kit. Why is that? Osteopontin binds to calcium oxalate. If calcium oxalate crystals are present in...	Assays
I see an 'Instrument not ready' error when clicking Run to analyze a plate. The camera cooling has not yet stabilized. Click OK in the error window, go to T...
I see Communication error when booting up DISCOVERY WORKBENCH The USB address information is lost or incorrect. Close DISCOVERY WORKBENCH, dis...	Instrument
If I have diluent or detection antibody reagents left over from a previous V-PLEX assay kit, can I use them in another V-PLEX kit? Check the reagent lot numbers, which are found in the certificate of analysis (C...	Assays
Installing Kit Layout Files (Add-ins) for DISCOVERY WORKBENCH To protect your assay analysis data you will need to be logged in as the Configu...	Software
Is a phospho-specific antibody always used to capture the phosphoprotein? The phospho-specific antibody may be the capture or the detection antibody with ...	Assays
Is an MSD instrument required to run MSD assays? Yes. The MSD platform is unique in its method for detection. As a result, MSD in...	Instrument Assays Plates
Is it necessary to use MSD GOLD Read Buffer with the R-PLEX singleplex assays? The R-PLEX multiplex assays use Read Buffer T. MSD GOLD Read Buffer is more convenient since it is provided at the typical work...	Assays
Is my information private? Absolutely. The personal and company information that you store on our site is n...	Ordering
Is my personal information safe? We value your trust. MSD’s network is secured and protected using Secure S...	Ordering
Is the lower limit of quantitation (LLOQ) in V-PLEX assays based on endogenous levels of the analyte? It is difficult to resolve an LLOQ if the endogenous levels of analytes in norma...	Assays
Is the same antibody pair used for V-PLEX kits and/or T-PLEX assays as used for R-PLEX sets for the same analyte? The antibody pairs are not necessarily the same. V-PLEX, U-PLEX, T-PLEX, S-PLEX ...	Assays
Is the validated Human Aß42 assay available with 4G8 detection? The Human Aß42 assay was developed and validated with 6E10 detection antib...	Assays
My instrument is showing an "Error 7461 Instrument not ready". What does this mean? The camera has not reached the proper temperature for operation. The SECTOR Imag...	Instrument
My online price is not the same as the quote I received. How do I ensure I get the right price? Occasionally, you will receive a special quote from your account manager. To ens...	Ordering
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Analysis of Results) Why do you recommend normalization to total protein mass per well rather th...	Assays
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Product info) Do the new total Akt and phospho-Akt (Ser473) assays use the same antibody...	Assays
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Protocol clarification and modification) 1. I would like to run my samples for the Phospho-Akt (Ser473) Kits and Total Ak...
Setting up ConfigurationManager for DISCOVERY WORKBENCH Start DISCOVERY WORKBENCH and check the status of “Role” at the bott...	Software
Should I avoid any reagents in my lysis buffer? High concentrations of ionic detergents (> 0.1%), urea (> 1 M), and other ...
Should I block the streptavidin plate before coating with capture antibodies? In some cases, blocking the plate prior to coating helps to reduce variability. ...	Assays
Should I choose a 2-fold or 4-fold dilution for my samples in the V-PLEX TH17 Panel 1 (human)? Sample dilution is dependent on the expected analyte levels in the samples. &nbs...	Assays
Should the control values fall within a certain range of set values? The lot-specific concentration of each analyte in the control pack is provided i...	Assays
The exported data format is incorrect. Can I correct the format? The data can be re-exported in correct format by clicking the Plate Data History...	Instrument
To conserve on sample volume, can you use 25 µl of sample in the validated Human Aß42 and Human Total Tau kits instead of 50 µl? We have validated the assays using 50 µl of sample (1:8 or 1:4 diluted). R...	Assays

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