| FAQ | Category | |
Are all products available online?
All catalog assays kits, assay consumables, assay development products, and inst... | Ordering | |
Are the Aß and Tau calibrators in the validated Human Aß42 and Human Total Tau kits native protein?
The Total Tau assay is a recombinant Tau 441 protein expressed in E.coli. The A&... | Assays
Ordering | |
Can I backup the WORKBENCH Software database?
Yes, the Workbench software comes with a backup utility that can be executed in ... | Instrument
Software | |
Can I cancel an order I placed online?
Yes, but cancellations are handled individually depending on the production or s... | Ordering | |
Can I combine R-PLEX and U-PLEX with Prototype and/or T-PLEX Assays?
Multiplexed U-PLEX and R-PLEX assays use Linkers to coat the plate with capture ... | | |
Can I conjugate proteins and antibodies with SULFO-TAG in my lab?
MSD provides SULFO-TAG NHS-Ester and you can follow the straightforward conjugat... | Assays | |
Can I denature the cell lysates?
Yes, ionic detergents or urea can be used to denature proteins; however, these r... | Assays | |
Can I develop my own phosphoprotein assays?
Yes, immobilizing antibodies on bare plates and labeling detection antibodies is... | Assays | |
Can I have my order shipped overnight?
All MSD orders within the continental US are shipped overnight at no additional ... | Ordering | |
Can I increase my sample volume to improve the sensitivity of the assay?
For the sample incubation step, most MSD assays use between 25-50 μL/well sam... | Assays | |
Can I move an MSD instrument myself?
Should your instrument need to be relocated, we can assist with the relocation a... | Instrument | |
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Akt Kits?
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Ak... | Assays | |
Can I order additional calibrators and/or detection antibodies?
Yes. Many calibrators and labeled secondary reagents are listed on our website u... | Reagents Diluents Buffers | |
Can I provide a quote number online with a order?
Yes, you can reference your quote number in the Special Instructions box du... | Ordering | |
Can I perform R-PLEX Singleplex assays on plates other than Small Spot Streptavidin plates?
R‑PLEX Singleplex assays can be developed on any MSD streptavidin- or avidin-coa... | Assays | |
Can I re-read a plate?
No, once the plate has been read and the wells have been 'fired' through the ECL... | Instrument | |
Can I re-read a plate?
No. Once the wells have been exposed to the electrochemiluminescence process, th... | Plates
Instrument
Software | |
Can I run my samples un-diluted to increase sensitivity?
For optimal performance, you should use the minimum sample dilution recommended ... | Assays | |
Can I run tissue homogenates or other sample types with V-PLEX kits?
MSD has not validated other sample types with the V-PLEX kits, but other sample ... | Assays | |
Can I save a shopping cart until my purchase order is ready?
Yes, your shopping cart will be saved automatically if you sign out or leave the... | Ordering | |
Can I use 384-well plates with R-PLEX?
You can use as little as 10 µL per well to coat a 384‑well plate. Calculat... | Assays | |
Can I use a different PC than the one supplied with the instrument?
No. Each computer MSD provides has been configured to a particular instrument so... | Software
Instrument | |
Can I use my own diluents and wash buffer when running R-PLEX assays?
Diluents used to generate the reported data are recommended as starting diluents... | Assays | |
Can I use my own diluents and wash buffer when running R-PLEX assays?
Diluents used to generate the reported data are recommended as starting diluents... | Assays | |
Can I use the same calibrator when testing sample types other than serum, plasma, or urine?
Yes, the same calibrator and thus the same calibration curve can be used for mul... | Assays | |
Can I view my order status online?
One of the benefits of placing all your orders online is the ability to see your... | Ordering | |
Can R-PLEX calibrators be purchased individually?
The R-PLEX calibrators are only available as part of an R-PLEX Antibody Set.... | Assays | |
Can sample dilutions other than the recommended dilutions be run? Can you lower the dilution factor to increase sensitivity?
Dilution linearity for CSF is provided in the product insert and data sheets. To... | Assays | |
Can the Aß42 assay be run in a homogenous format?
While the Aβ42 assay was validated with a washed assay, a homogenous protoc... | Assays | |
Can the assays be used with tissue lysates?
Yes, brain tissue homogenates, liver homogenates, homogenates from Matrigel plug... | Assays | |
Can the IL-8 HA assay be purchased as a singleplex?
No, the IL-8 HA is only available in select multiplex panels.... | Assays | |
Can the Validated Human Aß42 and Human Total Tau assays be multiplexed together or with the other Aß peptide assays?
The assays can be provided as a custom multiplex with comparable performance to ... | Assays | |
Can V-PLEX orders ship within the same day?
No. V-PLEX orders generally ship within 1-3 business days.... | Ordering
Assays | |
Can we network the MSD instrument computer?
If the system is under evaluation, it may not be networked. If the MSD system ha... | Instrument | |
Can we upgrade the Windows 2000 operating system to Windows XP?
Please contact MSD Instrument Service Support before upgrading to XP. This can b... | Instrument
Software | |
Can Windows updates be installed on the computer controlling the MSD instrument?
Many of our customers have installed Windows® updates without problems, incl... | Instrument
Software | |
Can you measure Aß42 levels in plasma with the validated Human Aß42 Kit?
The assay was validated with human CSF. MSD is currently working on blood-based ... | Assays | |
I see a 'Client already has a session' error when booting up DISCOVERY WORKBENCH.
This indicates that a duplicate of the DISCOVERY WORKBENCH software is running. ... | Instrument | |
Do I have to use the specific diluents provided on the R PLEX datasheet for my assay? What if I am multiplexing?
The Diluent Compatibility table on the R-PLEX datasheet lists the diluents that ... | Assays | |
Does MSD offer a multiplex for Aß42, Total Tau, and p-Tau (181)?
No, but the assays can be provided as a custom multiplex. However, the custom mu... | Assays | |
Does MSD offer the 12F4 antibody as a detection antibody with a 6E10 capture in the Human (6E10) Abeta 42 Ultra-Sensitive Kit?
No. We currently do not offer 12F4 as a detection antibody. We have not found an... | Assays | |
Does MSD use controls in the development of validated assays? If so, are they samples or diluted standards?
We test controls as part of the validation process for V‑PLEX assays. The t... | Assays | |
Does the Human Total Tau Kit work for mouse samples?
We have tested mouse brain lysates with the Human Total Tau Kit and were unable ... | Assays | |
FAQs for V-PLEX Proinflammatory Panel 1 (mouse) Kits
What is the difference between the 10-plex panel, a custom panel, and the s... | Assays | |
For R-PLEX assays, can I use less antibody to coat the plate?
R-PLEX is provided as a matched antibody set. We do not recommend using less of ... | | |
How are MSD assays validated?
A cell model that exhibits regulation of a phosphoprotein of interest is selecte... | | |
How are V-PLEX assays different from T-PLEX assays?
Unlike T-PLEX assays (tissue culture, ultra-sensitive, whole cell lysate, serum/... | Assays | |
How are your new Phospho-Akt (Ser473) and Total Akt Kits different than your existing kits?
Our new Phospho-Akt (Ser473) Kit (catalog# K150MND) and Total Akt Kit (catalog# ... | | |
How can I control cross-reactivity in multiplex assays?
MSD has several blockers available that can be added to lysis buffer or antibody... | Assays | |
How can I export each spot in 96- (384-) well format and do other data file customization?
Click Run to activate the Run Options window, check the Export Format Custom box... | Instrument | |