V-PLEX GLP-1 Active Kit Overview
The V-PLEX GLP-1 Active Kit is optimized for long-term and multi-site biomarker studies that require reliable, reproducible results with lot-to-lot consistency. The V-PLEX GLP-1 Active Kit enables the sensitive detection of native active GLP-1 in multiple sample types, including plasma and cell culture supernatants, across 5 species; human, mouse, rat, NHP and canine. To learn more about the advantages of V-PLEX assays
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All V-PLEX kits come with coated plates, calibrators, labeled detection antibodies, optimized diluents, a comprehensive product insert, and a certificate of analysis. V-PLEX Plus kits also include controls, wash buffer, and plate seals for added quality and convenience.
V-PLEX GLP-1 Active Assay Features
- Validated to measure Active GLP-1 in plasma and cell culture supernatants in 5 species; human, mouse, rat, NHP and canine
- Dynamic range: 0.02 – 120 pM
- LLOQ: 0.3 pM
Typical Data
Calibration curve for the V-PLEX GLP-1 Active Kit was assessed and representative data is presented below.
Figure 1. V-PLEX GLP-1 Active Kit Calibration Curve
GLP-1 Active Analyte
Description - Description - Glucagon-like peptide-1 (GLP-1), a post-translational product of preproglucagon, is a protein hormone produced in intestinal L cells that plays a key role in the promotion of glucose-dependent insulin secretion and insulin biosynthesis. GLP-1 works in concert with insulin to inhibit glucose secretion and lower overall blood glucose levels. The cleaved peptides, commonly referred to as GLP-1 (7-36) amide and GLP-1 (7-37) are the biologically active forms of GLP-1. In vivo, these active isoforms are rapidly cleaved by dipeptidyl peptidase IV (DPPIV). The primary amino acid sequence for GLP-1 is conserved among mammalian species, i.e. human, mouse, rat, monkey, canine, etc.
Disease Research Area - Metabolic Diseases
Application Area - Metabolic, Cardiovascular
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Specifications
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Kit Contents
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Documentation
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Application(s)
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Metabolic
,
Cardiovascular
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Analyte(s)
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GLP-1 (active)
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Species
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Human, Mouse, Rat, Non-human primate, Canine
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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mouse monoclonal
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Detection Antibody
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mouse monoclonal
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Dynamic Range
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0.02 – 120 pM
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Sample Type
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Cell Culture Supernatant, EDTA Plasma, Plasma, Rodent Plasma
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Recombinant standards
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synthetic peptide
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LLOD (Sensitivity)
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0.02 pM
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Condition
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Multi-Component
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Gene ID(s)
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2641, 14526, 24952, 403571, 102134567
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UniProt ID(s)
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P01275, P55095, P06883, P29794, A0A0D9RSY0
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 vial
(75 μL)
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GLP-1 (active) SECTOR Plate
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2-8°C
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1 ea
(1 ea)
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Diluent Assembly 13 (includes Diluent 12 - 10 mL, and Diluent 13 - 10 mL)
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≤-10°C
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1 ea
(1 ea)
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RT
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1 ea
(18 mL)
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Aprotinin, 50 uL
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2-8°C
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1 vial
(50 μL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 vial
(1 vial)
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2-8°C
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1 vial
(375 μL)
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GLP-1 (active) SECTOR Plate
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2-8°C
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5 ea
(1 ea)
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Diluent Assembly 14 (includes Diluent 12 - 50 mL, and Diluent 13 - 50 mL)
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≤-10°C
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1 ea
(1 ea)
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RT
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1 ea
(90 mL)
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Aprotinin, 250 uL
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2-8°C
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1 vial
(250 μL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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25 vial
(1 vial)
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2-8°C
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5 vial
(375 μL)
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GLP-1 (active) SECTOR Plate
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2-8°C
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25 ea
(1 ea)
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Diluent Assembly 14 (includes Diluent 12 - 50 mL, and Diluent 13 - 50 mL)
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≤-10°C
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5 ea
(1 ea)
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RT
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5 ea
(90 mL)
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Aprotinin, 250 uL
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2-8°C
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5 vial
(250 μL)
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Product Inserts
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Certificate of analysis
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SDS
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters