Profiling of sputum inflammatory mediators in asthma and chronic obstructive pulmonary disease.

Bafadhel M, McCormick M, Saha S, McKenna S, Shelley M, Hargadon B, Mistry V, Reid C, Parker D, Dodson P, Jenkins M, Lloyd A, Rugman P, Newbold P, Brightling CE.
Journal   Respiration.
Species  
Analytes Measured   Eotaxin , I-TAC , IL-5 , IL-6 , IL-6R , IL-8 , IP-10 , MCP-1 , MCP-4 , MMP-8 , MMP-9 , RANTES , TARC , TNF-RI , TNF-RII , Flt-1 VEGFR1
Matrix Tested   Sputum
Year   2012
Volume   83
Page Numbers   36-44
Application   Cytokines and Chemokines
Abstract
BACKGROUND: Asthma and chronic obstructive pulmonary disease (COPD) display features of overlap in airway physiology and airway inflammation. Whether inflammatory phenotypes in airway disease describe similar mediator expression is unknown.

OBJECTIVES: To explore the relationship of airway inflammation and cytokine and chemokine expression in asthma and COPD.

METHODS: Subjects with asthma and COPD (n = 54 and n = 49) were studied. Clinical characteristics and sputum were collected at entry into the study. A 2-step sputum processing method was performed for supernatant and cytospin preparation. Meso Scale Discovery and Luminex platforms were used to measure cytokines, chemokines and matrix metalloproteinase levels.

RESULTS: Analytes sensitive to dithiothreitol (DTT) that had increased recovery in the 2-step sputum process were IL-1β, 4, 5, 10, 13, IFN-γ, TNFRI, GM-CSF, CCL2, 3, 4, 5, 13 and 17. There was a differential expression in IL-8, TNFRI and TNFRII between asthma and COPD [mean fold difference (95% CI): IL-8, 2.6 (1.3-5.4), p = 0.01; TNFRI, 2.1 (1.3-5.4), p = 0.03; TNFRII, 2.6 (1.2-5.6), p = 0.02]. In neutrophilic and eosinophilic airway inflammation, TNFα, TNFRI, TNFRII, IL-6, IL-8 and IL-5 could differentiate between these phenotypes. However, these phenotypes were unrelated to the diagnosis of asthma or COPD.

CONCLUSION: Recovery of sputum mediators sensitive to DTT can be improved using the described sputum processing technique. Within airway inflammatory sub-phenotypes there is a differential pattern of mediator expression that is independent of disease. Whether these inflammatory phenotypes in asthma and COPD confer distinct pathogeneses, therapeutic responses and clinical phenotypes needs to be further evaluated.

View Publications

Related Products

U-PLEX Long COVID Combo 1 (human)
IFN-β, IFN-γ, IL-6, Pentraxin 3 | Human
Multiplex
V-PLEX Chemokine Panel 1 (human) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
Multiplex
V-PLEX Human TARC Gen. B Kit
TARC | Human
Singleplex
V-PLEX Human MCP-1 Gen. B Kit
MCP-1 | Human
Singleplex
Human MCP-4 Antibody
MCP-4 | Human
Human MCP-1 Antibody
MCP-1 | Human
Human IP-10 Antibody
IP-10 | Human
Human Eotaxin Antibody
Eotaxin | Human
Human TARC Antibody
TARC | Human
V-PLEX Plus NHP MCP-1 Gen. B Kit
MCP-1 | Non-human primate
Singleplex
V-PLEX NHP MCP-1 Gen. B Kit
MCP-1 | Non-human primate
Singleplex
V-PLEX NHP MCP-4 Gen. B Kit
MCP-4 | Non-human primate
Singleplex
V-PLEX NHP Eotaxin Gen. B Kit
Eotaxin | Non-human primate
Singleplex
V-PLEX Human MCP-4 Gen. B Kit
MCP-4 | Human
Singleplex
V-PLEX Human Eotaxin Gen. B Kit
Eotaxin | Human
Singleplex
V-PLEX Chemokine Panel 1 (NHP) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Non-human primate
Multiplex
V-PLEX Plus NHP Eotaxin Gen. B Kit
Eotaxin | Non-human primate
Singleplex
V-PLEX Plus Human TARC Gen. B Kit
TARC | Human
Singleplex
V-PLEX Plus Human MCP-4 Gen. B Kit
MCP-4 | Human
Singleplex
V-PLEX Plus Human MCP-1 Gen. B Kit
MCP-1 | Human
Singleplex
V-PLEX Plus Human Eotaxin Gen. B Kit
Eotaxin | Human
Singleplex
V-PLEX Plus Chemokine Panel 1 (NHP) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Non-human primate
Multiplex
V-PLEX NHP TARC Gen. B Kit
TARC | Non-human primate
Singleplex
V-PLEX Plus NHP TARC Gen. B Kit
TARC | Non-human primate
Singleplex
V-PLEX Plus NHP MCP-4 Gen. B Kit
MCP-4 | Non-human primate
Singleplex
V-PLEX Plus Chemokine Panel 1 (human) Gen. B Kit
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
Multiplex
V-PLEX Plus Human IP-10 Gen. B Kit
IP-10 | Human
Singleplex
V-PLEX NHP IP-10 Gen. B Kit
IP-10 | Non-human primate
Singleplex
V-PLEX Human IP-10 Gen. B Kit
IP-10 | Human
Singleplex
V-PLEX Plus NHP IP-10 Gen. B Kit
IP-10 | Non-human primate
Singleplex
Chemokine Panel 1 (human) Gen. B Control Pack
Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC | Human
R-PLEX Mouse I-TAC Assay
I-TAC | Mouse
Singleplex
R-PLEX Mouse I-TAC Antibody Set
I-TAC | Mouse
R-PLEX Human MMP-9 (active) Assay
MMP-9 (active) | Human
Singleplex
R-PLEX Human MMP-9 (active) Antibody Set
MMP-9 (active) | Human
U-PLEX CAR-T Cell Safety Combo 1 (human)
GM-CSF, IFN-γ, IL-1β, IL-6, IL-10, MCP-1, TNF-α | Human
Multiplex
U-PLEX TBI Combo 1 (human)
CD40L (soluble), Fractalkine, IFN-γ, IL-1β, IL-6, IL-18, MCP-1, RANKL/TNFSF11, TNF-α, VEGF-D | Human
Multiplex
U-PLEX Multiple Sclerosis Combo 1 (human)
CTACK, Fractalkine, GRO-α, IL-7, IL-12/IL-23p40, IP-10, MCP-1, MIP-1β, MIP-3α | Human
Multiplex
U-PLEX Alzheimer's Disease Combo 1 (human)
Eotaxin, IL-1α, IL-12/IL-23p40, IL-16, IL-17A, MCP-1, MDC, MIF, SDF-1α, YKL-40 | Human
Multiplex
U-PLEX Human TNF-RI Assay
TNF-RI | Human
Singleplex
U-PLEX Human TNF-RI 384-well Assay
TNF-RI | Human
Singleplex
U-PLEX Human RANTES Assay
RANTES | Human
Singleplex
U-PLEX Human RANTES 384-well Assay
RANTES | Human
Singleplex
U-PLEX Human TNF-RII Assay
TNF-RII | Human
Singleplex
U-PLEX Human TNF-RII 384-well Assay
TNF-RII | Human
Singleplex
U-PLEX Human TNF-RII Antibody Set
TNF-RII | Human
U-PLEX Human TNF-RI Antibody Set
TNF-RI | Human
U-PLEX Human RANTES Antibody Set
RANTES | Human
U-PLEX Human VEGFR-1/Flt-1 Antibody Set
VEGFR-1/Flt-1 | Human
U-PLEX Human VEGFR-1/Flt-1 Assay
VEGFR-1/Flt-1 | Human
Singleplex
U-PLEX Human VEGFR-1/Flt-1 384-well Assay
VEGFR-1/Flt-1 | Human
Singleplex
U-PLEX Oncolysis Combo 1 (human)
CTLA-4, IFN-α2a, IFN-β, IL-1β, IL-2Rα, IL-6, IL-8, IP-10, LAG3, MIP-1α, PD1 (epitope 1), TIGIT | Human
Multiplex
U-PLEX Metastasis Combo 1 (human)
E-Selectin, MMP-1, MMP-2, MMP-7, MMP-9 (total), P-Selectin, VEGFR-1/Flt-1 | Human
Multiplex
U-PLEX Macrophage M1 Combo 1 (mouse)
IL-1β, IL-6, IL-12p70, IL-15, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Mouse
Multiplex
U-PLEX Custom Biomarker Group 1 (mouse) 384-well Assays
Mouse
Multiplex
U-PLEX Mouse Eotaxin 384-Well Assay
Eotaxin | Mouse
Singleplex
U-PLEX Mouse IL-6 384-Well Assay
IL-6 | Mouse
Singleplex
U-PLEX Mouse IL-5 384-Well Assay
IL-5 | Mouse
Singleplex
U-PLEX Mouse MCP-1 384-Well Assay
MCP-1 | Mouse
Singleplex
U-PLEX Mouse IP-10 384-Well Assay
IP-10 | Mouse
Singleplex
U-PLEX Mouse TARC 384-Well Assay
TARC | Mouse
Singleplex
U-PLEX Mouse RANTES 384-Well Assay
RANTES | Mouse
Singleplex
U-PLEX NHP Eotaxin 384-Well Assay
Eotaxin | Non-human primate
Singleplex
U-PLEX Mouse TNF-RI 384-Well Assay
TNF-RI | Mouse
Singleplex
U-PLEX NHP IL-8 384-Well Assay
IL-8 | Non-human primate
Singleplex
U-PLEX NHP IL-6 384-Well Assay
IL-6 | Non-human primate
Singleplex
U-PLEX NHP IL-5 384-Well Assay
IL-5 | Non-human primate
Singleplex
U-PLEX NHP MCP-1 384-Well Assay
MCP-1 | Non-human primate
Singleplex
U-PLEX NHP I-TAC 384-Well Assay
I-TAC | Non-human primate
Singleplex
U-PLEX NHP IP-10 384-Well Assay
IP-10 | Non-human primate
Singleplex
U-PLEX NHP TARC 384-Well Assay
TARC | Non-human primate
Singleplex
U-PLEX Custom Metabolic Group 1 (mouse) 384-well Assays
BAFF, BCA-1/BLC, BDNF, CD40/TNFRSF5, C-Peptide, Eotaxin, EPO, FGF-21, Ghrelin (active), Ghrelin (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27p28/IL-30, IL-31, IL-33, Insulin, IP-10, KC/GRO, Leptin, MCP-1, MCP-5/CCL12, MDC, MIP-1α, MIP-1β, MIP-2, MIP-3α, MMP-9 (total), PYY (total), RANTES, TARC, TNF-α, VEGF-A | Mouse
Multiplex
U-PLEX Biomarker Group 1 (NHP) 384-well Assays
CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, FLT3L, Fractalkine, G-CSF, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17F, IL-18, IL-22, IL-23, IP-10, I-TAC, MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-3α, MIP-3β, MIP-5, SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, VEGF-A, YKL-40 | Non-human primate
Multiplex
R-PLEX Mouse VEGFR-1/Flt-1 Assay
VEGFR-1/Flt-1 | Mouse
Singleplex
R-PLEX Mouse VEGFR-1/Flt-1 Antibody Set
VEGFR-1/Flt-1 | Mouse
U-PLEX Human MCP-1 384-Well Assay
MCP-1 | Human
Singleplex
U-PLEX Human Eotaxin 384-Well Assay
Eotaxin | Human
Singleplex
U-PLEX Human IL-8 384-Well Assay
IL-8 | Human
Singleplex
U-PLEX Human IL-6 384-Well Assay
IL-6 | Human
Singleplex
U-PLEX Human IL-5 384-Well Assay
IL-5 | Human
Singleplex
U-PLEX Human I-TAC 384-Well Assay
I-TAC | Human
Singleplex
U-PLEX Human IP-10 384-Well Assay
IP-10 | Human
Singleplex
U-PLEX Human TARC 384-Well Assay
TARC | Human
Singleplex
U-PLEX Custom Biomarker Group 1 (human) 384-well Assays
CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FLT3L, Fractalkine, G-CSF, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, IP-10, I-TAC, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-1β, MIP-3α, MIP-3β, MIP-5, SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
U-PLEX Custom Metabolic Group 1 (human) 384-well Assays
BAFF, BDNF, β-NGF, C-Peptide, CTACK, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, EPO, FGF-21, FGF-23, FLT3L, Fractalkine, FSH, G-CSF, Ghrelin (active), Ghrelin (total), GIP (active), GIP (inactive), GIP (total), GLP-1 (active), GLP-1 (inactive), GLP-1 (total), Glucagon, GM-CSF, GRO-α, I-309, IFN-α2a, IFN-β, IFN-γ, IL-1α, IL-1β, IL-1RA, IL-2, IL-2Rα, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17D, IL-17E/IL-25, IL-17F, IL-18, IL-21, IL-22, IL-23, IL-27, IL-29/IFN-λ1, IL-31, IL-33, Insulin, IP-10, Leptin, Luteinizing Hormone (LH), MCP-1, MCP-2, MCP-4, M-CSF, MDC, MIF, MIP-1α, MIP-5, PP, Proinsulin, PYY (total), SDF-1α, TARC, TNF-α, TNF-β, TPO, TRAIL, TSLP, VEGF-A, YKL-40 | Human
Multiplex
Human MCP-4 Antibody
MCP-4 | Human
S-PLEX Proinflammatory Panel 1 (human) Kit
IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, TNF-α | Human
Multiplex
U-PLEX Macrophage M2 Combo 1 (mouse)
IL-4, IL-10, IL-13, MDC, TARC | Mouse
Multiplex
S-PLEX NHP IL-6 Kit
IL-6 | Non-human primate
Singleplex
S-PLEX NHP IL-5 Kit
IL-5 | Non-human primate
Singleplex
S-PLEX Human IL-6 Kit
IL-6 | Human
Singleplex
S-PLEX Human IL-5 Kit
IL-5 | Human
Singleplex
U-PLEX Macrophage M2 Combo 1 (human)
Eotaxin-2, IL-4, IL-10, IL-13, M-CSF, MDC, TARC | Human
Multiplex
U-PLEX Macrophage M1 Combo 1 (human)
IL-1β, IL-6, IL-12p70, IL-18, IL-23, IP-10, MCP-1, MIP-1α, TNF-α | Human
Multiplex
V-PLEX Plus Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Plus Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
V-PLEX Viral Panel 3 NHP Kit
IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10 | Non-human primate
Multiplex
V-PLEX Viral Panel 3 Human Kit
IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α | Human
Multiplex
U-PLEX Viral Combo 1 (NHP)
G-CSF, GM-CSF, IFN-α2a, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Non-human primate
Multiplex
U-PLEX Viral Combo 1 (human)
G-CSF, GM-CSF, IFN-α2a, IFN-β, IFN-γ, IL-1β, IL-1RA, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP-1, MIP-1α, TNF-α, VEGF-A | Human
Multiplex
Product Search
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters