p53

p53 (protein 53) is a transcription factor and tumor suppressor protein with an apparent molecular weight of 53 kDa that plays a critical role in cell cycle regulation, progression, and apoptosis. p53 is composed of an amino terminal transcription activation domain, a proline rich domain critical to the p53 apoptotic functions, a central DNA binding domain, a nuclear localization sequence, and an oligomerization domain. The extent of DNA damage and different types of cellular stress determine the set of downstream targets activated by p53. MDM2 is a potent negative regulator of p53 through its binding and subsequent polyubiquitination of p53, resulting in proteasome dependent degradation. This negative regulation can be relieved both through phosphorylation of p53, resulting in destabilization of the MDM2-p53 interaction, and through phosphorylation and ubiquitination of MDM2. p53 is the most commonly mutated gene in cancer, and a functional copy of p53 is required to maintain a non-tumorigenic phenotype. When cell repair is possible, p53 activates genes that pause the cell cycle allowing time for DNA repair, but when damage is extensive, p53 activates the BCL-2 family of proteins leading to apoptosis. p53’s role as a transcription factor and the negative regulation of the protein by MDM2 mediated polyubiquitination has been extensively researched due to its crucial role in cancer prevention and cell cycle control.

 

p53 Singleplex Products

Our p53 immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of p53. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.

 

View All
p53 Multiplex Products

Our multiplex panels let you process up to 10 analytes simultaneously in each well of a MULTI-SPOT microplate. They provide the same outstanding performance as our singleplex immmunoassays while saving money, sample, and time.

 

View All
Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

  • Phospho-p53 -
  • Ub-p53 -
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters