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U-PLEX Kidney Injury Combo 1 (human)

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U-PLEX Combos offer popular panels of biomarkers that have been grouped together for convenient ordering. They have been tested for multiplex compatibility and are fulfilled as a kit. The U-PLEX Kidney Injury Combo 1 (human) combines assays for 3 biomarkers related to kidney damage (Table 1) that are in Biomarker Group 3. The assays can be used for quantitative measurements in the sample types listed in the "Specifications" tab below, and offer detection limits (LLODs) in the sub- to low pg/mL range.

Table 1. List of analytes and typical detection limits

Analyte	LLOD - ULOD	units
A2M	13.2 - 32,000	pg/mL
Clusterin	57.7 - 500,000	pg/mL
Cystatin C	23.9 - 20,000	pg/mL
NGAL/LCN2	0.78 - 4,000	pg/mL
RBP4	3.78 - 4,000	pg/mL
Serpin A1	5.52 - 22,000	pg/mL

Specifications
Kit Contents
Documentation
References

Application(s)		Kidney Injury , Toxicology
Analyte(s)		A2M, Clusterin, Cystatin C, NGAL/LCN2, RBP4, Serpin A1
Species		Human
Plate Type		96-well
U-PLEX Group Compatibility		Biomarker Group 3
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Sample Type		Cell Culture Media, Plasma, Serum
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K15389K-1

Product Description

Storage

Quantity per Kit

Kidney Injury Combo 1, Box 1 of 2

Multi-Component

1 each
(1 plate size)

U-PLEX Linker Set, 3-Assay (300 µL)	2-8 °C	1 each (1 vial)
U-PLEX Human Clusterin Antibody Set	2-8 °C	1 each (1 plate size)
U-PLEX Human Cystatin C Antibody Set	2-8 °C	1 each (1 plate size)
U-PLEX Human NGAL/LCN2 Antibody Set	2-8 °C	1 each (1 plate size)
Human Clusterin Calibrator	2-8 °C	1 vial (1 vial)
Calibrator 25	2-8 °C	1 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)
Diluent 100	2-8 °C	1 bottle (200 mL)
U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)
Diluent 12	≤-10 °C	1 bottle (50 mL)
Diluent 11	≤-10 °C	1 bottle (10 mL)
MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Kidney Injury Combo 1, Box 2 of 2

Multi-Component

1 each
(1 plate size)

U-PLEX Linker Set, 3-Assay (300 µL)	2-8 °C	1 each (1 vial)
U-PLEX Human A2M Antibody Set	2-8 °C	1 each (1 plate size)
U-PLEX Human RBP4 Antibody Set	2-8 °C	1 each (1 plate size)
U-PLEX Human Serpin A1 Antibody Set	2-8 °C	1 each (1 plate size)
Calibrator 26	2-8 °C	1 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)
Diluent 100	2-8 °C	1 bottle (200 mL)
U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)
Diluent 12	≤-10 °C	1 bottle (50 mL)
Diluent 11	≤-10 °C	1 bottle (10 mL)
MSD GOLD Read Buffer B	RT	1 bottle (18 mL)

Kit Contents for Catalog No. K15389K-2

Product Description

Storage

Quantity per Kit

Kidney Injury Combo 1, Box 1 of 2

Multi-Component

1 each
(5 plate size)

U-PLEX Linker Set, 3-Assay (1.8 mL)	2-8 °C	1 each (1 vial)
U-PLEX Human Clusterin Antibody Set	2-8 °C	1 each (5 plate size)
U-PLEX Human Cystatin C Antibody Set	2-8 °C	1 each (5 plate size)
U-PLEX Human NGAL/LCN2 Antibody Set	2-8 °C	1 each (5 plate size)
Human Clusterin Calibrator	2-8 °C	5 vial (1 vial)
Calibrator 25	2-8 °C	5 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)
Diluent 100	2-8 °C	3 bottle (200 mL)
U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)
Diluent 12	≤-10 °C	3 bottle (50 mL)
Diluent 11	≤-10 °C	1 bottle (50 mL)
MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Kidney Injury Combo 1, Box 2 of 2

Multi-Component

1 each
(5 plate size)

U-PLEX Linker Set, 3-Assay (1.8 mL)	2-8 °C	1 each (1 vial)
U-PLEX Human A2M Antibody Set	2-8 °C	1 each (5 plate size)
U-PLEX Human RBP4 Antibody Set	2-8 °C	1 each (5 plate size)
U-PLEX Human Serpin A1 Antibody Set	2-8 °C	1 each (5 plate size)
Calibrator 26	2-8 °C	5 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)
Diluent 100	2-8 °C	3 bottle (200 mL)
U-PLEX Stop Solution	2-8 °C	1 bottle (40 mL)
Diluent 12	≤-10 °C	3 bottle (50 mL)
Diluent 11	≤-10 °C	1 bottle (50 mL)
MSD GOLD Read Buffer B	RT	1 bottle (90 mL)

Kit Contents for Catalog No. K15389K-4

Product Description

Storage

Quantity per Kit

Kidney Injury Combo 1, Box 1 of 2

Multi-Component

5 each
(25 plate size)

U-PLEX Linker Set, 3-Assay (1.8 mL)	2-8 °C	5 each (1 vial)
U-PLEX Human Clusterin Antibody Set	2-8 °C	5 each (5 plate size)
U-PLEX Human Cystatin C Antibody Set	2-8 °C	5 each (5 plate size)
U-PLEX Human NGAL/LCN2 Antibody Set	2-8 °C	5 each (5 plate size)
Human Clusterin Calibrator	2-8 °C	25 vial (1 vial)
Calibrator 25	2-8 °C	25 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)
Diluent 100	2-8 °C	3 bottle (200 mL)
Diluent 100	2-8 °C	2 bottle (1000 mL)
U-PLEX Stop Solution	2-8 °C	5 bottle (40 mL)
Diluent 12	≤-10 °C	3 bottle (200 mL)
Diluent 11	≤-10 °C	5 bottle (50 mL)
MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Kidney Injury Combo 1, Box 2 of 2

Multi-Component

1 each
(25 plate size)

U-PLEX Linker Set, 3-Assay (1.8 mL)	2-8 °C	5 each (1 vial)
U-PLEX Human A2M Antibody Set	2-8 °C	5 each (5 plate size)
U-PLEX Human RBP4 Antibody Set	2-8 °C	5 each (5 plate size)
U-PLEX Human Serpin A1 Antibody Set	2-8 °C	5 each (5 plate size)
Calibrator 26	2-8 °C	25 vial (1 vial)
U-PLEX 3-Assay, 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)
Diluent 100	2-8 °C	3 bottle (200 mL)
Diluent 100	2-8 °C	2 bottle (1000 mL)
U-PLEX Stop Solution	2-8 °C	5 bottle (40 mL)
Diluent 12	≤-10 °C	3 bottle (200 mL)
Diluent 11	≤-10 °C	5 bottle (50 mL)
MSD GOLD Read Buffer B	RT	5 bottle (90 mL)

Kit Contents for Catalog No. K15389K-21

Product Description	Storage	Quantity per Kit
Kidney Injury Combo 1 (hu), Box 1 of 2 (1 PL)	Multi-Component	1 each (1 each)

Kidney Injury Combo 1 (hu), Box 2 of 2 (1 PL)	Multi-Component	1 each (1 each)

Kit Contents for Catalog No. K15389K-22

Product Description	Storage	Quantity per Kit
Kidney Injury Combo 1 (hu), Box 1 of 2 (5 PL)	Multi-Component	1 each (5 each)

Kidney Injury Combo 1 (hu), Box 2 of 2 (5 PL)	Multi-Component	1 each (5 each)

Kit Contents for Catalog No. K15389K-24

Product Description	Storage	Quantity per Kit
Kidney Injury Combo 1 (hu), Box 1 of 2 (25 PL)	Multi-Component	5 each (25 each)

Kidney Injury Combo 1 (hu), Box 2 of 2 (25 PL)	Multi-Component	1 each (25 each)

Order Details

K15389K

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K15389K-1

1 plate size

$2,455.00

Not Applicable

K15389K-2

5 plate size

$9,819.00

Not Applicable

K15389K-4

25 plate size

$44,185.00

Not Applicable

K15389K-21

1 plate size

$2,254.00

Usually ships in 1-3 days

K15389K-22

5 plate size

$9,819.00

Usually ships in 1-3 days

K15389K-24

25 plate size

$44,185.00

Usually ships in 1-3 days

Customize

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		U-PLEX Biomarker Group 3 (human) Multiplex Insert
Datasheets		U-PLEX Human A2M U-PLEX Human Clusterin U-PLEX Human Cystatin C U-PLEX Human NGAL/LCN2 U-PLEX Human RBP4 U-PLEX Human Serpin A1
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 10, Diluent 11, Diluent 12, Diluent 13, Diluent 15, Diluent 16 and Diluent 17 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer B U-PLEX Linker Set
Scientific Posters		Development and Validation of Multiplexed Human Kidney Biomarker Assay Panels

Title	Journal	Year
Urinary biomarkers track the progression of nephropathy in hypertensive and obese rats. Zhang Q, Davis KJ, Hoffmann D, Vaidya VS, Brown RP, Goering PL.	Biomark Med.	2014
Assessment of cisplatin-induced kidney injury using an integrated rodent platform. Chen Y, Brott D, Luo W, Gangl E, Kamendi H, Barthlow H, Lengel D, Fikes J, Kinter L, Valentin JP, Bialecki R.	Toxicol Appl Pharmacol	2013
Early prediction of polymyxin-induced nephrotoxicity with next generation urinary kidney injury biomarkers. Keirstead ND, Wagoner MP, Bentley P, Blais M, Brown C, Cheatham L, Ciaccio P, Dragan Y, Ferguson D, Fikes J, Galvin M, Gupta A, Hale M, Johnson N, Luo W, McGrath F, Pietras M, Price S, Sathe AG, Sasaki JC, Snow D, Walsky RL, Kern G.	Toxicol Sci.	2013
Ozone induces glucose intolerance and systemic metabolic effects in young and aged brown Norway rats. Bass V, Gordon CJ, Jarema KA, Macphail RC, Cascio WE, Phillips PM, Ledbetter AD, Schladweiler MC, Andrews D, Miller D, Doerfler DL, Kodavanti UP.	Toxicol Appl Pharmacol.	2013
Susceptibility of adult and senescent Brown Norway rats to repeated ozone exposure: an assessment of behavior, serum biochemistry and cardiopulmonary function. Gordon CJ, Jarema KA, Lehmann JR, Ledbetter AD, Schladweiler MC, Schmid JE, Ward WO, Kodavanti UP, Nyska A, MacPhail RC.	Inhal Toxicol	2013
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U-PLEX Kidney Injury Combo 1 (human)

Kit Contents for Catalog No. K15389K-1

Kit Contents for Catalog No. K15389K-2

Kit Contents for Catalog No. K15389K-4

Kit Contents for Catalog No. K15389K-21

Kit Contents for Catalog No. K15389K-22

Kit Contents for Catalog No. K15389K-24

Order Details

K15389K

S 600

SQ 120

Q 60

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