Mild oxidative damage in the diabetic rat heart is attenuated by glyoxalase-1 overexpression.

Brouwers O, de Vos-Houben JMJ, Niessen PMG, Miyata T, van Nieuwenhoven F, Janssen BJA, Hageman G, Stehouwer CDA, Schalkwijk CG.
Journal   Int. J. Mol. Sci.
Species  
Analytes Measured   Troponin I cardiac , Troponin T cardiac , FABP3 , Myl3
Matrix Tested   Plasma
Year   2013
Volume   14
Page Numbers   15724-15739
Application   Cardiac
Abstract
Diabetes significantly increases the risk of heart failure. The increase in advanced glycation endproducts (AGEs) and oxidative stress have been associated with diabetic cardiomyopathy. We recently demonstrated that there is a direct link between AGEs and oxidative stress. Therefore, the aim of the current study was to investigate if a reduction of AGEs by overexpression of the glycation precursor detoxifying enzyme glyoxalase-I (GLO-I) can prevent diabetes-induced oxidative damage, inflammation and fibrosis in the heart. Diabetes was induced in wild-type and GLO-I transgenic rats by streptozotocin. After 24-weeks of diabetes, cardiac function was monitored with ultrasound under isoflurane anesthesia. Blood was drawn and heart tissue was collected for further analysis. Analysis with UPLC-MSMS showed that the AGE Nε-(1-carboxymethyl)lysine and its precursor 3-deoxyglucosone were significantly elevated in the diabetic hearts. Markers of oxidative damage, inflammation, and fibrosis were mildly up-regulated in the heart of the diabetic rats and were attenuated by GLO-I overexpression. In this model of diabetes, these processes were not accompanied by significant changes in systolic heart function, i.e., stroke volume, fractional shortening and ejection fraction. This study shows that 24-weeks of diabetes in rats induce early signs of mild cardiac alterations as indicated by an increase of oxidative stress, inflammation and fibrosis which are mediated, at least partially, by glycation.

Related Products

Cardiac Injury Panel 3 Rat Kit
FABP3/H-FABP, Myl3/CMLC1, Troponin I (cardiac), Troponin T (cardiac) | Rat
Multiplex
R-PLEX Human Troponin T Cardiac Antibody Set
Troponin T (cardiac) | Human
R-PLEX Human FABP3/H-FABP Antibody Set
FABP3/H-FABP | Human
Human FABP3 Kit
FABP3/H-FABP | Human
Singleplex
Muscle Injury Panel 3 Mouse Kit
FABP3/H-FABP, Myl3/CMLC1, Troponin I (cardiac), Troponin I (fast-twitch) | Mouse
Multiplex
Muscle Injury Panel 1 Rat Kit
FABP3/H-FABP, Myl3/CMLC1, Troponin I (cardiac), Troponin I (fast-twitch), Troponin T (cardiac) | Rat
Multiplex
Rat Cardiac Troponin ITC Complex Kit
Troponin I (cardiac), Troponin ITC Complex | Rat
Singleplex
Cardiac Injury Panel 2 Rat Kit
FABP3/H-FABP, Troponin I (cardiac), Troponin T (cardiac) | Rat
Multiplex
Browse Our Products

By Analytes
By Applications
Search
Customer Service/Orders


Scientific/Technical Support


Instrument Support


Company Headquarters