ELISA Comparison
Developing novel immunoassays using MSD’s platform is fast and easy, and transferring existing ELISAs can often be accomplished with minimal optimization in less than 2 days.
Our
MULTI-ARRAY technology provides inherently better sensitivity and a broader dynamic range than ELISAs. This means fewer samples fall below the assay detection range.
Compared to ELISA technology, MSD assays:
- Require far less sample
- Provide more sensitive and precise results
- Handle complex biological matrices easily
- Use simpler, faster protocols
- Quantify up to 10 analytes per well
- Measure elevated and normal expression samples without multiple dilutions
Unlike standard ELISA plate readers, MSD instruments provide increased throughput with fast read times (1–3 minutes per plate) and no downtime for cleaning or user maintenance.
| |
ELISA |
MSD |
| Sample volume requirement |
50–100 μL (per analyte) |
10–25 μL (up to 10 analytes) |
| Multiplex panels |
No |
10 analytes simultaneously |
| Flexible panel configuration |
No |
Yes (U-PLEX) |
| Dynamic range |
1–2 logs |
3–4+ logs |
| Matrix effects |
Yes |
Greatly reduced |
| Simple protocols |
No |
Yes |
| Wash steps |
Many |
Typically 1–3 |
| Read times |
Slow |
Up to 960 results in 1 to 3 min |
| Rapid throughput |
No |
Yes |
| Instrument maintenance |
Daily cleaning and calibration |
No user maintenance required |
*Kruse N, Schulz-Schaeffer WJ, Schlossmacher MG, Mollenhauer B. Development of electrochemiluminescence-based singleplex and multiplex assays for the quantification of α-synuclein and other proteins in cerebrospinal fluid. Methods. 2012;56:514-8.
For more information on converting an existing ELISA to the MSD platform, contact an account manager.
For more information on how our technology compares to others, download our technology brochure.