Scientific Frequently Asked Question

Results 101 - 150 of 150 for FAQ

FAQ	Category
V-PLEX and V-PLEX Plus Questions Does the V stand for validated? V-PLEX products are MSD's...	Assays
What amount (micrograms) of lysate should I use? The performance of the assay will depend greatly on the abundance of the protein...	Assays
What are kit layout files? The first step in data analysis is identifying the spot location of each assay. ...	Software
What are the dimensions of your 96- and 384-well plates? Please see immunoassay plates for plate-specific information....	Plates
What are the MSD Product Purchase Terms & Conditions? When purchasing products or services from MSD, you are agreeing to our Term...	Ordering
What are the release specifications (QC) for the kits? All of our validated kits come with a Certificate of Analysis (COA) that documen...	Assays
What browsers are supported by the MSD website? To ensure you have the best experience possible, we recommend using the followin...
What do I need to purchase along with the R-PLEX and/or U-PLEX Antibody Sets to perform a multiplex assay? Multiplex assays, using either R-PLEX antibody sets or a combination of R-PLEX a...	Assays
What do I need to purchase along with the R-PLEX Antibody Set to perform a singleplex assay? The R-PLEX Antibody Set includes a matched antibody set and calibrator. MSD GOLD...	Assays
What is the best way to thaw MSD reagents that have been shipped frozen? For diluents (use at room temperature): Option 1: The day befor...	Reagents Diluents Buffers
What is the binding capacity of avidin and streptavidin plates? For Standard plates, the avidin or streptavidin-coated electrode surface is capa...
What is the difference between an R-PLEX Antibody Set and a U-PLEX Antibody Set? R-PLEX Antibody Sets contain a biotinylated capture antibody, a SULFO-TAG conjug...	Assays
What is the difference between anti-hu IL-8 and IL-8 (HA) detection antibodies? The anti-hu IL-8 (HA) antibody has been validated and is recommended when high I...	Assays
What is the difference between V-PLEX and V-PLEX Plus kits? The V-PLEX Plus kits include additional items (controls, wash buffer, and plate ...	Assays
What is the MSD Lysis Buffer formulation? 150 mM NaCl 20 mM Tris, pH 7.5 1 mM EDTA 1 mM EGTA 1% T...	Assays
What is the MSD wash buffer formulation? The MSD wash buffer is a 20-fold concentrated phosphate buffered solution with s...
What is the recommended reagent formulation for conjugation? Carrier proteins (e.g. BSA and gelatin) and buffer additives such as glycerol, d...	Reagents Diluents Buffers
What is the sample volume required for the cytokine 30-plex or the 40-plex V-PLEX kits? The sample volume required depends on sample type and dilution factor used. Reco...	Assays
What is the shelf life of the Human Aß42 and Human Total Tau kits? Plates expire 15 months from the date of manufacture. Kit expiration dates are b...	Assays
What is the turnaround time for orders? Catalog Items Our catalog assays generally ship within 1-3 business days. Cust...	Assays Ordering
What other lysis buffers have been used with MSD assays? Various modified RIPA buffers with low concentrations of ionic detergents and ur...	Assays Reagents Diluents Buffers
What parameters can I optimize for my particular sample? End users can vary the following parameters: Concentration of detection a...	Assays
What plates do I purchase for R-PLEX multiplex assays? R-PLEX multiplex assays use 10-spot 96-well U‑PLEX plates, available as U-PLEX D...	Assays
What should I do if I have less than 25 µL of sample? A minimum of 25 µL of solution is required to prevent the plate surface fr...	Assays
What should I do if the read buffer is added to the plate, but the instrument is not available? If the instrument is not available, MSD recommends that you remove the read buff...	Assays Reagents Diluents Buffers
What standards are used to calibrate the assays? The assays are developed using positive and negative control Whole Cell Lysate S...	Assays
What type of payments does MSD accept? MSD accepts payments via purchase order. You may enter this information during t...	Ordering
What volume of lysate should I use? A minimum of 25 µL should be placed in each well to ensure adequate covera...	Assays
When I add more lysate to increase assay signals, why do the signals continue to decrease? High concentration of proteins in the sample can inhibit antibody binding, espec...
Where can I find general information about MSD assay development and plate spotting? The MSD Immunoblot and General Notes technical presentation includes detailed in...	Assays
Where can I find information about immunogenicity assay development? This product insert provides detailed information about using MSD Bridging ...	Assays
Where can I find the calibrator/standard curve concentrations for V-PLEX assays? A certificate of analysis (COA) is provided with each V-PLEX kit lot including t...	Assays
Where do I enter our tax exempt status? Tax exempt status and/or tax IDs are not required during checkout. We manage thi...	Ordering
Which diluent should I use for CSF samples? Diluent 35 is the best option as it closely mimics the sample matrix (it is esse...	Assays
Who do I contact for MSD’s Custom Conjugation Services? Please contact the MSD Customer Service Department at customerservice@mesos...
Why are my unknown sample values being reported as N/A when the signal is within standard curve range? As long as standards are assigned first, controls and unknowns will automaticall...	Software
Why are the lot numbers for my controls different from the ones shown in the certificate of analysis (COA) for my V-PLEX kit? The MSD control packs are not specific to a particular V-PLEX kit lot. Thi...	Assays
Why are the singleplex validated assays on a 4-spot plate? The performance of a small-spot plate and 4-spot plate are equivalent. We are wo...	Assays
Why did I get a message that my data has been quarantined? How do I avoid this message? Your data may be quarantined if the barcode reader fails to read the plate barco...	Software
Why do I need to dilute my CSF samples for Aß42, I usually use undiluted CSF? Other kits also use undiluted samples. We determined that a 1:8 dilution of CSF provided the best combination of matrix...	Assays
Why do I need to register? Registration is required in order for you to see customer-specific pricing, plac...	Ordering
Why do I not see the same dynamic range as I see with cytokine assays? Non-specific binding of antibodies, especially phospho-specific antibodies, limi...	Assays
Why do only some of the V-PLEX assays have NIBSC/WHO reference information? NIBSC/WHO standards are not available for all analytes. Results are included in ...	Assays
Why do the calibrator curve concentrations change from one lot to the next for V-PLEX assays? Calibrators are re-evaluated with each new V-PLEX production lot to provide opti...	Assays
Why do the concentrations of the highest calibrators in the certificate of analysis (COA) for validated kits vary between kit lots? As part of the kit lot verification process, we compare the calibrator for the n...	Assays
Why is the Human Aß42 Kit LLOD given as a range? Should I expect a mid-range LLOD value? The LLOD was calculated across many runs. Since the LLOD is computed as 2.5 stan...	Assays
Why was Wash Buffer not included in my V-PLEX assay kit? Wash Buffer is only included with the “V-PLEX Plus” version of the k...	Assays Reagents Diluents Buffers
Will the new tau calibrator be incorporated into the other Tau assays? MSD is currently working on new calibrators for our existing Total Tau and phosp...	Assays
Will the validated Alzheimer's kits be provided with controls? MSD sells quality controls as a separate package (Neurodegeneration Control Pack...	Assays
Will the validated Human Aß42 and Human Total Tau kits work with other sample types? The assay was validated with human CSF. The kits have been run with tissue cultu...

Results 101 - 150 of 150 for FAQ

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