V-PLEX Chemokine Gen. B Assays

V-PLEX: Chemokine Gen. B Assays.  Improved, Reproducible, and Validated


V-PLEX: MSD’s Validated Assay Platform

V-PLEX validated assays deliver reproducibility and reliability to support the most demanding long-term studies. V-PLEX assays maximize consistency in results and confidence in data. Click here to learn more about our V-PLEX assay development, validation, manufacturing, and quality control processes.

Biomarker Assays for Inflammatory Response and Immune System Regulation Studies

Studying the expression profile of chemokines is crucial for gaining insights into their roles in regulating the migration of monocytes, neutrophils, and lymphocytes. Research on chemokines plays a pivotal role in understanding the immune system and its diverse response to antigens and various disease states, such as cancer, inflammatory diseases, and allergic reactions.

MSD has introduced the latest Generation B (Gen. B) version of the V-PLEX Chemokine Panel 1 (human) and (NHP) kits to support investigations into chemotactic cytokines (chemokines). The V-PLEX Chemokine Panel 1 Gen. B kits use new reagents and protocols, ensuring precise and reproducible results consistent across multiple lots.

Key Improvements
  • New Human and NHP Eotaxin assays
  • New Human and NHP MIP-1α assays
  • Improved sensitivity for most assays
  • Shortened protocol – no read buffer incubation step
  • New assay diluent
  • Ready-to-use MSD GOLD Read Buffer B

Assays in the V-PLEX Chemokine Panel 1 Gen. B kits are available as validated individual assays or as a part of a validated multiplex panel (Tables 1 and 2). Assays have a wide dynamic range and provide greater sensitivity, as shown in the table below (Table 2).

Elevate your cytokine and chemokine research with the quality and performance you expect from MSD.


Click the button to create your custom multiplex panels or subset multiplexes from a pre-configured panel.

Table 1. Multiplex V-PLEX Chemokine Panel 1 Gen. B Kits
V-PLEX Kits V-PLEX Cat. # V-PLEX Plus Cat. # Analytes
Chemokine Panel 1 (human) Gen. B K15705D K15705G Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC
Chemokine Panel 1 (NHP) Gen. B K15712D K15712G Eotaxin, Eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC

Table 2. Individual Assays in the V-PLEX Chemokine Panel 1 Gen. B Kits
Analytes LLOQ
V-PLEX Cat. # (human) V-PLEX Plus Cat. #
V-PLEX Cat. #
V-PLEX Plus Cat. #
Eotaxin 2.14 0.20 - 910 K151ANGD K151ANGG K156ANGD K156ANGG
Eotaxin-3 11.1 1.44 - 11,100 K151ANJD K151ANJG K156ANJD K156ANJG
IP-10 1.29 0.12 - 1,240 K151ANLD K151ANLG K156ANLD K156ANLG
MCP-1 1.45 0.16 - 247 K151ANPD K151ANPG K156ANPD K156ANPG
MCP-4 0.518 0.05 - 234 K151ANRD K151ANRG K156ANRD K156ANRG
MDC 6.43 1.00 - 2,930 K151ANQD K151ANQG K156ANQD K156ANQG
MIP-1α 0.357 0.05 - 403 K151ANMD K151ANMG K156ANMD K156ANMG
MIP-1β 1.20 0.09 - 390 K151ANHD K151ANHG K156ANHD K156ANHG
TARC 2.14 0.11 - 481 K151ANKD K151ANKG K156ANKD K156ANKG

All V-PLEX kits come with coated plates, calibrators, individual labeled detection antibodies, optimized diluents, a comprehensive product insert, and a certificate of analysis. V-PLEX Plus kits also include controls, wash buffer, and plate seals for added quality and convenience.