PARP

Poly (ADP-ribose) polymerase (PARP) is a highly abundant nuclear enzyme involved in DNA repair that synthesizes poly ADP-ribose from NAD+ in response to damaged DNA. When cells are exposed to a critical level of damaging agents, cellular apoptosis is induced through an intracellular signaling cascade. Activation of the caspase family of apoptosis-specific proteases results in the cleavage of PARP by caspase-3. Cleavage of PARP produces the N-terminal 24 kDa DNA binding domain fragment, and the C-terminal 89 kDa catalytic domain fragment, rendering it inactive. The 24 kDa fragment has been shown to retain its DNA binding affinity for strand breaks, thereby inhibiting further DNA repair, ADP-ribose polymer formation, and transcription. Caspase-7 is also involved in PARP cleavage during apoptosis. High levels of PARP activity have been implicated in a caspase-independent cell death pathway through the depletion of cellular energy stores (NAD+) and the downstream effector AIF (Apoptosis-Inducing Factor).

 

PARP Singleplex Products

Our PARP immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of PARP. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.

 

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PARP Multiplex Products

Our multiplex panels let you process up to 10 analytes simultaneously in each well of a MULTI-SPOT microplate. They provide the same outstanding performance as our singleplex immmunoassays while saving money, sample, and time.

 

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Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

  • PARP (Clv) -
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