ERK-1/2

ERK (extracellular signal regulated kinases) 1 and 2, are proline-directed serine/threonine protein kinases, also known as p44 MAPK (mitogen-activated protein kinase) and p42 MAPK, respectively. These closely-related kinase isoforms, identified by their molecular weight, are activated by various extracellular signals including growth factors, cytokines, hormones, and neuro-transmitters. The activation occurs through the phosphorylation of threonine 202 and tyrosine 204 of ERK1 and threonine 185 and tyrosine 187 of ERK2 by the upstream kinases MEK1 and MEK2. The upstream kinases receive their activation signal through receptors (receptor tyrosine kinases or integrins) via a pathway involving adaptor proteins, guanine nucleotide exchange factors, small GTP binding proteins, and MAPKKs. Activated ERK1/2 phosphorylates targets in both the cytosol and the nucleus. Cytoplasmic substrates for ERK include SOS, MNK1/2, and the 90 kDa ribosomal protein S6 kinases (RSKs). Nuclear translocation of activated ERK affects gene expression and DNA replication by the phosphorylation of MSK 1 and 2 and the transcription factors Elk-1, Sap1, and Sap2.

 

ERK-1/2 Singleplex Products

Our ERK-1/2 immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of ERK-1/2. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.

 

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ERK-1/2 Multiplex Products

Our multiplex panels let you process up to 10 analytes simultaneously in each well of a MULTI-SPOT microplate. They provide the same outstanding performance as our singleplex immmunoassays while saving money, sample, and time.

 

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Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

  • Phospho-ERK1/2 -
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