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The Phospho/Total ERK1/2 Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-ERK1/2 (Thr202/Tyr204; Thr185/Tyr187) and total ERK1/2 in human, mouse, and rat whole cell lysates.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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ERK-1/2 (total)
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
,
MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Phospho-ERK1/2: Mouse Monoclonal
Total ERK1/2: Mouse Monoclonal
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Detection Antibody
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Phospho-ERK1/2: Mouse Monoclonal
Total ERK1/2: Mouse Monoclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3002.15.0100
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Total ERK1/2 Antibody
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2-8 °C
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1 vial
(1 vial)
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Phosphatase Inhibitor I 0.1 mL
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2-8 °C
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1 each
(0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8 °C
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1 each
(1 each)
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SDS, 0.6 mL
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RT
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1 each
(1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 0.05 mL
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≤-10 °C
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1 each
(1 each)
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Phospho/Total ERK1/2 Plate
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2-8 °C
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1 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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MSD Blocker D-R, 0.05 mL
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≤-10 °C
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1 vial
(1 vial)
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Total ERK1/2 Antibody
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2-8 °C
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1 vial
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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SDS, 0.6 mL
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RT
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 0.250 mL
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≤-10 °C
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1 each
(1 each)
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Phospho/Total ERK1/2 Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Description
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Storage
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Quantity per Kit
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SULFO-TAG Anti-Total ERK1/2 Antibody
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2-8 °C
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4 vial
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Phosphatase Inhibitor I 2.0mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8 °C
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1 each
(1 each)
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SDS, 0.6 mL
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RT
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4 each
(1 each)
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Protease Inhibitor Solution 2.0mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 1.0 mL
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≤-10 °C
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1 each
(1 each)
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Phospho/Total ERK1/2 Plate
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2-8 °C
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20 each
(1 each)
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2-8 °C
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1 bottle
(200 mL)
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2-8 °C
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1 bottle
(1000 mL)
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RT
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1 bottle
(200 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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4 vial
(0.2 mL)
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Product Inserts
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Datasheets
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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A Fully Human, Allosteric Monoclonal Antibody That Activates the Insulin Receptor and Improves Glycemic Control.
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Analytical approach to characterize the intratumoral pharmacokinetics and pharmacodynamics of gefitinib in a glioblastoma model.
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Dependence of Wilms tumor cells on signaling through insulin-like growth factor 1 in an orthotopic xenograft model targetable by specific receptor inhibition.
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Rationale-based engineering of a potent long-acting FGF21 analog for the treatment of type 2 diabetes
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Transcriptional activation of inflammasome components by Libby amphibole and the role of iron.
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