Caspase-3 (Apopain, CPP32) is part of a family of cysteinyl aspartate-specific proteases (caspases) that cleave target proteins after aspartate residues. Many caspases are involved in apoptosis signaling within the cell and are categorized as initiator or effector caspases. Caspases become activated through dimerization and proteolysis of procaspases, forming an active enzyme which contains two large and two small subunits. Caspase-3 is an effector caspase that is activated through cleavage by ("by" or "of") caspase-9, an initiator caspase activated through cytochrome c release from the mitochondria, ATP, and an interaction with Apaf-1. Active caspase-3 has been shown to cleave many proteins including Bcl-2, PARP, p21, Akt, DNA-PK, 14-3-3 proteins, and eukaryotic translation initiation factors 4G and 2α. Cleavage of caspase-3 substrates facilitates apoptosis through suppression of cell survival pathways or the amplification of intracellular apoptotic signals.


Caspase-3 Singleplex Products

Our Caspase-3 immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of Caspase-3. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.


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Caspase-3 Multiplex Products

Our multiplex panels let you process up to 10 analytes simultaneously in each well of a MULTI-SPOT microplate. They provide the same outstanding performance as our singleplex immmunoassays while saving money, sample, and time.


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Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

  • Caspase-3 (Clv) -
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