COVID-19/SARS-CoV-2 Research Applications

COVID-19

MSD's V-PLEX Serology Panels for COVID-19 are now available for sale. The release includes the V-PLEX SARS-CoV-2 Panel 2, which Operation Warp Speed (OWS) has chosen as the basis of its standard binding assays for immunogenicity assessments in all funded Phase III clinical trials of vaccines. The serology tests are provided as complete kits, including the same reference standards and controls that will be used in OWS laboratories to analyze clinical trial samples, making it possible for OWS scientists to directly compare quantitative results between different clinical trials. We offer multiplex serology panels that can be used to detect isotypes (IgG, IgM, and IgA) of antibodies to important antigens related to SARS-CoV-2, SARS-1, MERS, circulating Coronaviruses, and other respiratory diseases. The products also include kits to measure antibodies that block binding of ACE2 to SARS-CoV-2 Spike and RBD, a high throughput alternative to traditional neutralization assays.

The V-PLEX Serology products are built from a menu of sixteen (16) antigens, organized into panels. The SARS-CoV-2 panels focus on antigens from the SARS-CoV-2 virus. The Coronavirus panels combine the SARS-CoV-2 panels with selected antigens from SARS-1, MERS, the circulating Coronaviruses and Influenza. The Respiratory panels are the most comprehensive with additional antigens for Influenza and RSV.

We also offer multiplex assays to measure key biomarkers that are involved in many inflammatory pathways. Individuals infected by SARS-CoV-2 show elevated levels of inflammatory cytokines that could lead to cytokine release syndrome or "cytokine storm." You can easily configure other panels from our full menu.


For Research Use Only. Not for use in diagnostic procedures.

 


V-PLEX Serology Panels Catalog Number Antigens
V-PLEX SARS-CoV-2 Panel 1 Kit
(IgG, IgM, IgA)
K15359U (IgG)
K15360U (IgM)
K15361U (IgA)
SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX SARS-CoV-2 Panel 2 Kit
(IgG, IgM, IgA)
K15383U (IgG)
K15384U (IgM)
K15385U (IgA)
SARS-CoV-2 N, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 1 Kit
(IgG, IgM, IgA)
K15362U (IgG)
K15363U (IgM)
K15364U (IgA)
Flu A/Hong Kong H3, HCoV-HKU1 Spike, HCoV-OC43 Spike, MERS-CoV Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 2 Kit
(IgG, IgM, IgA)
K15369U (IgG)
K15370U (IgM)
K15371U (IgA)
HCoV-229E Spike, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 3 Kit
(IgG, IgM, IgA)
K15399U (IgG)
K15400U (IgM)
K15401U (IgA)
HCoV-229E Spike, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, MERS-CoV Spike, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX Respiratory Panel 1 Kit
(IgG, IgM, IgA)
K15365U (IgG)
K15366U (IgM)
K15367U (IgA)
Flu A/Hong Kong H3, Flu A/Michigan H1, Flu A/Shanghai H7, Flu B/Brisbane HA, Flu B/Phuket HA, RSV Pre-Fusion F
V-PLEX COVID-19 Respiratory Panel 2 Kit
(IgG, IgM, IgA)
K15372U (IgG)
K15373U (IgM)
K15374U (IgA)
HCoV-229E Spike, Flu A/Hong Kong H3, Flu A/Michigan H1, Flu A/Shanghai H7, Flu B/Brisbane HA, Flu B/Phuket HA, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, RSV Pre-Fusion F, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Respiratory Panel 3 Kit
(IgG, IgM, IgA)
K15403U (IgG)
K15404U (IgM)
K15405U (IgA)
HCoV-229E Spike, Flu A/Hong Kong H3, Flu A/Michigan H1, Flu A/Shanghai H7, Flu B/Brisbane HA, Flu B/Phuket HA, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, RSV Pre-Fusion F, SARS-CoV-1 Spike, SARS-CoV-2 N, MERS-CoV Spike, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX SARS-CoV-2 384 Panel 1 Kit
(IgG, IgM, IgA)
K25392U (IgG)
K25393U (IgM)
K25394U (IgA)
SARS-CoV-2 N, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike

V-PLEX Neutralization Panels Catalog Number Antigens
V-PLEX SARS-CoV-2 Panel 1 Kit
(ACE2)
K15375U SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX SARS-CoV-2 Panel 2 Kit
(ACE2)
K15386U SARS-CoV-2 N, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 1 Kit
(ACE2)
K15376U Flu A/Hong Kong H3, HCoV-HKU1 Spike, HCoV-OC43 Spike, MERS-CoV Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 2 Kit
(ACE2)
K15378U HCoV-229E Spike, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Coronavirus Panel 3 Kit
(ACE2)
K15402U HCoV-229E Spike, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, SARS-CoV-1 Spike, SARS-CoV-2 N, MERS-CoV Spike, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Respiratory Panel 2 Kit
(ACE2)
K15379U HCoV-229E Spike, Flu A/Hong Kong H3, Flu A/Michigan H1, Flu A/Shanghai H7, Flu B/Brisbane HA, Flu B/Phuket HA, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, RSV Pre-Fusion F, SARS-CoV-1 Spike, SARS-CoV-2 N, SARS-CoV-2 S1 NTD, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX COVID-19 Respiratory Panel 3 Kit
(ACE2)
K15406U HCoV-229E Spike, Flu A/Hong Kong H3, Flu A/Michigan H1, Flu A/Shanghai H7, Flu B/Brisbane HA, Flu B/Phuket HA, HCoV-HKU1 Spike, HCoV-OC43 Spike, HCoV-NL63 Spike, RSV Pre-Fusion F, SARS-CoV-1 Spike, SARS-CoV-2 N, MERS-CoV Spike, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike
V-PLEX SARS-CoV-2 384 Panel 1 Kit
(ACE2)
K25395U SARS-CoV-2 N, SARS-CoV-2 S1 RBD, SARS-CoV-2 Spike

S-PLEX Antigen Detection Assay Catalog Number Analyte
S-PLEX SARS-CoV-2 N Kit K150ADHS SARS-CoV-2 N

R-PLEX Antigen Detection Antibody Sets Catalog Number Analyte
R-PLEX SARS-CoV-2 N Antibody Set F20ADC SARS-CoV-2 N
R-PLEX SARS-CoV-2 Spike Antibody Set F20ADB SARS-CoV-2 Spike

V-PLEX Biomarker Panels Catalog Number Analytes
V-PLEX Viral Panel 1 Human Kit K15345D IL-1β, IL-6, IL-8, TNF-α
V-PLEX Viral Panel 2 Human Kit K15346D IFN-γ, IL-1β, IL-4, IL-6, IL-8, IL-10, TNF-α
V-PLEX Viral Panel 3 Human Kit K15347D IFN-γ, IL-1β, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, TNF-α
V-PLEX Proinflammatory Panel 1 Human Kit K15049D IFN-γ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, TNF-α
V-PLEX Vascular Injury Panel 2 Human Kit K15198D CRP, ICAM-1, SAA, VCAM-1
V-PLEX Human Biomarker 54-Plex Kit K15248D CRP, Eotaxin, Eotaxin-3, FGF (basic), VEGFR-1/Flt-1, GM-CSF, ICAM-1, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-8 (HA), IL-9, IL-10, IL-12/IL-23p40, IL-12p70, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17B, IL-17C, IL-17D, IL-1RA, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, MIP-3α, PlGF, SAA, TARC, Tie-2, TNF-α, TNF-β, TSLP, VCAM-1, VEGF-A, VEGF-C, VEGF-D
V-PLEX Viral Panel 1 NHP Kit K15348D IL-1β, IL-6, IL-8
V-PLEX Viral Panel 2 NHP Kit K15349D IFN-γ, IL-1β, IL-6, IL-8, IL-10
V-PLEX Viral Panel 3 NHP Kit K15350D IFN-γ, IL-1β, IL-5, IL-6, IL-8, IL-10
V-PLEX Proinflammatory Panel 1 NHP Kit K15056D IFN-γ, IL-1β, IL-2, IL-6, IL-8, IL-10

U-PLEX Combos Catalog Number Analytes
U-PLEX Viral Combo 1 Human K15343K G-CSF, GM-CSF, IFN-α2a, IFN-β, IFN-γ, IL-1RA, IL-1β, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP1, MIP-1α, TNF-α, VEGF-A
U-PLEX Cytokine Combo 1 Human K15045K GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A
U-PLEX Viral Combo 1 NHP K15344K G-CSF, GM-CSF, IFN-α2a, IFN-γ, IL-1RA, IL-1β, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IP-10, MCP1, MIP-1α, TNF-α, VEGF-A
U-PLEX Cytokine Combo 1 NHP K15057K GM-CSF, IL-1α, IL-5, IL-7, IL-12/IL-23p40, IL-15, IL-16, IL-17A, TNF-β, VEGF-A

S-PLEX Assays Catalog Number Analyte
S-PLEX Human G-CSF Kit K151K3S G-CSF
S-PLEX Human GM-CSF Kit K151F3S GM-CSF
S-PLEX Human IFN-α2a Kit K151P3S IFN-α2a
S-PLEX Human IL-4 Kit K151A3S IL-4
S-PLEX Human IL-6 Kit K151B3S IL-6
S-PLEX Human IL-10 Kit K151Y2S IL-10
S-PLEX Human TNF-α Kit K151E3S TNF-α

R-PLEX Antibody Set Catalog Number Analyte
R-PLEX Human Ferritin Antibody Set F21ADA Ferritin

References

  1. Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses
  2. Evaluation of a novel multiplexed assay for determining IgG levels and functional activity to SARS-CoV-2
  3. Humoral Response Dynamics Following Infection with SARS-CoV-2
  4. Evaluation of the mRNA-1273 Vaccine against SARS-CoV-2 in Nonhuman Primates
  5. Safety and immunogenicity of the ChAdOx1 nCoV-19 vaccine against SARS-CoV-2: a preliminary report of a phase 1/2, single-blind, randomised controlled trial
  6. Correlation of SARS-CoV-2 nucleocapsid antigen and RNA concentrations in nasopharyngeal samples from children and adults using an ultrasensitive and quantitative antigen assay
  7. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China
  8. Pathogenic human coronavirus infections: causes and consequences of cytokine storm and immunopathology
  9. Coronavirus infections and immune responses
  10. Handbook of COVID-19 Prevention and Treatment

SARS-CoV-2 image courtesy of CDC/ Alissa Eckert, MS; Dan Higgins, MAMS