V-PLEX Plus Human IL-23 Kit Overview
The V-PLEX Plus Human IL-23 Kit is optimized for long-term and multi-site biomarker studies that require reliable, reproducible results with lot-to-lot consistency. The V-PLEX Plus Human IL-23 Kit enables the sensitive detection of native IL-23 in multiple sample types including serum, plasma, urine, cell culture supernatants, and PBMCs. To learn more about the advantages of V-PLEX assays click here.
The V-PLEX Plus Human IL-23 assay is available as an individual assay kit as well as in a multiplex combination with other TH17-related analytes in the V-PLEX TH17 Panel 1 (human) Kit (catalog # K15085G, K15085D).
All V-PLEX kits come with coated plates, calibrators, labeled detection antibodies, optimized diluents, a comprehensive product insert, and a certificate of analysis. V-PLEX Plus kits also include controls, wash buffer, and plate seals for added quality and convenience.
V-PLEX Plus Human IL-23 Assay Features
- Validated to measure human IL-23 in serum, plasma, cell culture supernatants, PBMCs, and urine
- Dynamic range: 0.274 pg/mL – 3,250 pg/mL
- LLOQ: 4.60 pg/mL
Typical Data
Calibration curve accuracy and precision for the V-PLEX Plus Human IL-23 assay were assessed and representative data are presented below.
Figure 1. V-PLEX Plus Human IL-23 Calibration Curve
Figure 2. V-PLEX Plus Human IL-23 Data
IL-23 Analyte
Description - Interleukin-23 (IL-23) is a heterodimeric cytokine that is structurally and functionally related to IL 12. Both share the 40 kDa (p40) subunit of IL-12, and this forms a disulfide link with a 19 kDa (p19) subunit to make the active IL-23 molecule. IL-23 is produced by activated macrophages and dendritic cells in response to certain bacterial and viral pathogens and may play a key role in sustaining inflammatory responses that link innate and adaptive immunity. The main role of IL-23 involves the differentiation of TH17 cells, a novel subset of CD4+ memory T cells, and their stimulation to produce the cytokine, IL-17. IL-23 is a key player in sustaining cellular immunity by promoting the survival and effector cytokine production of Th1 memory cells.
Disease Focus - Rheumatoid arthritis, inflammatory bowel disease, diabetes, and tumor growth.
Application Area - Inflammation, cytokines and chemokines
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Specifications
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Kit Contents
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References
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Documentation
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-23
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Goat Polyclonal
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Dynamic Range
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0.274 - 3,250 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant, Urine
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LLOD (Sensitivity)
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0.274 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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TH17 Panel 1 (human) Control 1
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2-8°C
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1 vial
(NA vial)
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TH17 Panel 1 (human) Control 2
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2-8°C
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1 vial
(NA vial)
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TH17 Panel 1 (human) Control 3
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2-8°C
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1 vial
(NA vial)
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 vial
(75 μL)
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Human IL-23 SECTOR Plate
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2-8°C
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1 ea
(1 ea)
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Diluent Assembly 3 (includes Diluent 3 - 5 mL, and Diluent 43 - 10 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(100 mL)
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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TH17 Panel 1 (human) Control 1
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2-8°C
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5 vial
(NA vial)
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TH17 Panel 1 (human) Control 2
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2-8°C
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5 vial
(NA vial)
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TH17 Panel 1 (human) Control 3
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2-8°C
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5 vial
(NA vial)
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2-8°C
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5 vial
(1 vial)
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2-8°C
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1 vial
(375 μL)
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Human IL-23 SECTOR Plate
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2-8°C
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5 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(100 mL)
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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TH17 Panel 1 (human) Control 1
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2-8°C
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25 vial
(NA vial)
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TH17 Panel 1 (human) Control 2
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2-8°C
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25 vial
(NA vial)
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TH17 Panel 1 (human) Control 3
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2-8°C
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25 vial
(NA vial)
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2-8°C
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25 vial
(1 vial)
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2-8°C
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5 vial
(375 μL)
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Human IL-23 SECTOR Plate
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2-8°C
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25 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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5 ea
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RT
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5 ea
(100 mL)
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RT
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5 ea
(50 mL)
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Title
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Journal
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Year
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Structural basis for the dual recognition of IL-12 and IL-23 by ustekinumab.
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Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations.
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Product Inserts
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Product Highlights
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Certificate of analysis
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SDS
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Scientific Posters
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters