Angiogenesis Panel 1 (human) measures 7 biomarkers which are proangiogenic proteins: VEGF-A, VEGF-C, VEGF-D, Tie-2, Flt-1, PlGF, and FGF (basic). Angiogenesis is essential to the the process of neovascularization under both physiological conditions and pathological conditions, including cancer, diabetic retinopathy, rheumatoid arthritis, and ischemia. Angiogenesis is modulated by initiatory and inhibitory molecules. Angiogenic markers are potential prognostic markers for disease activity and indicators of response to chemotherapy. Several different strategies have been designed to target vascular endothelial growth factor receptor (Flt-1/VEGFR) signal transduction. Several VEGF-neutralizing antibodies have been successful in combination with chemotherapy in limiting angiogenesis and tumor progression. Other strategies to target the VEGF/Flt-1 pathway include soluble VEGFRs (Traps), receptor tyrosine-kinase inhibitors that target VEGFR2/Flk-1, and neutralizing aptamers. The Angiogenesis Panel 1 (human) Kit provides the assay-specific components for the quantitative determination of VEGF-A, VEGF-C, VEGF-D, Tie-2, Flt-1, PlGF, and FGF (basic). This kit may be used for the analysis of all or any combination of these biomarkers in human serum, plasma, cell supernatant, cerebral spinal fluid, urine, and cell lysate.
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Specifications
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Kit Contents
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Documentation
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References
Application(s)
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Growth Factors
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Cardiovascular
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Analyte(s)
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FGF (basic), PlGF, Tie-2, VEGF-A, VEGF-C, VEGF-D, VEGFR-1/Flt-1
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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VEGF-A: Mouse Monoclonal VEGF-C: Mouse Monoclonal VEGF-D: Mouse Monoclonal Tie-2: Mouse Monoclonal Flt-1: Mouse Monoclonal PlGF: Mouse Monoclonal FGF (basic): Mouse Monoclonal
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Detection Antibody
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VEGF-A: Mouse Monoclonal VEGF-C: Goat Polyclonal VEGF-D: Goat Polyclonal Tie-2: Goat Polyclonal Flt-1: Goat Polyclonal PlGF: Goat Polyclonal FGF (basic): Mouse Monoclonal
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LLOD (Sensitivity)
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VEGF-A: 0.48 pg/mL VEGF-C: 11.1 pg/mL VEGF-D: 2.53 pg/mL Tie-2: 21.1 pg/mL Flt-1: 0.56 pg/mL PlGF: 0.32 pg/mL FGF (basic): 0.08 pg/mL
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Dynamic Range
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VEGF-A: 0.48–1510 pg/mL VEGF-C: 11.1–17 500 pg/mL VEGF-D: 2.53–18 800 pg/mL Tie-2: 21.1–63 400 pg/mL Flt-1: 0.56–6410 pg/mL PlGF: 0.32–2370 pg/mL FGF (basic): 0.08–1780 pg/mL
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Sample Type
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Serum, EDTA Plasma, Urine, Cell Culture Supernatant, Cell Lysate
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Recombinant standards
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VEGF-A: Expressed in Sf21 insect cells VEGF-C: Expressed in murine myeloma cells VEGF-D: Expressed in Sf21 insect cells Tie-2: Expressed in murine myeloma cells Flt-1: Expressed in Sf21 insect cells PlGF: Expressed in E.coli FGF (basic): Expressed in E.coli
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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Angiogenesis Control 1
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≤-70 °C
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1 vial (1 vial)
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Angiogenesis Control 2
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≤-70 °C
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1 vial (1 vial)
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Angiogenesis Control 3
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≤-70 °C
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1 vial (1 vial)
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≤-70 °C
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1 vial (1 vial)
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2-8 °C
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1 vial (75 μL)
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SULFO-TAG Human VEGF Antibody
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2-8 °C
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1 vial (75 μL)
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2-8 °C
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1 vial (75 μL)
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2-8 °C
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1 vial (75 μL)
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2-8 °C
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1 vial (75 μL)
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2-8 °C
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1 vial (75 μL)
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2-8 °C
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1 vial (75 μL)
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Angiogenesis Panel 1 (human) Plate
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2-8 °C
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1 each (1 each)
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Diluent Assembly 11 (includes Diluent 7 – 50 mL, Diluent 11 – 5 mL)
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≤-10 °C
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1 each
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RT
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1 bottle (100 mL)
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RT
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1 bottle (50 mL)
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MSD Blocker A
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RT
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1 bottle (250 mL)
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MSD Phosphate Buffer 5X
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RT
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1 bottle (50 mL)
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Product Description
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Storage
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Quantity per Kit
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Angiogenesis Control 1
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≤-70 °C
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5 vial (1 vial)
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Angiogenesis Control 2
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≤-70 °C
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5 vial (1 vial)
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Angiogenesis Control 3
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≤-70 °C
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5 vial (1 vial)
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≤-70 °C
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5 vial (1 vial)
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2-8 °C
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1 vial (375 μL)
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SULFO-TAG Human VEGF Antibody
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2-8 °C
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1 vial (375 μL)
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2-8 °C
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1 vial (375 μL)
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2-8 °C
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1 vial (375 μL)
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2-8 °C
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1 vial (375 μL)
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2-8 °C
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1 vial (375 μL)
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2-8 °C
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1 vial (375 μL)
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Angiogenesis Panel 1 (human) Plate
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2-8 °C
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5 each (1 each)
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Diluent Assembly 12 (includes Diluent 7 – 50 mL, Diluent 11 – 50 mL)
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≤-10 °C
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1 each
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RT
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1 bottle (100 mL)
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RT
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1 bottle (50 mL)
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MSD Blocker A
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RT
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1 bottle (250 mL)
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MSD Phosphate Buffer 5X
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RT
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1 bottle (50 mL)
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Product Description
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Storage
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Quantity per Kit
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Angiogenesis Control 1
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≤-70 °C
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25 vial (1 vial)
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Angiogenesis Control 2
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≤-70 °C
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25 vial (1 vial)
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Angiogenesis Control 3
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≤-70 °C
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25 vial (1 vial)
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≤-70 °C
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25 vial (1 vial)
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2-8 °C
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5 vial (375 μL)
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SULFO-TAG Human VEGF Antibody
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2-8 °C
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5 vial (375 μL)
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2-8 °C
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5 vial (375 μL)
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2-8 °C
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5 vial (375 μL)
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2-8 °C
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5 vial (375 μL)
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2-8 °C
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5 vial (375 μL)
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2-8 °C
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5 vial (375 μL)
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Angiogenesis Panel 1 (human) Plate
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2-8 °C
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25 each (1 each)
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Diluent Assembly 12 (includes Diluent 7 – 50 mL, Diluent 11 – 50 mL)
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≤-10 °C
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5 each
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RT
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5 bottle (100 mL)
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RT
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5 bottle (50 mL)
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MSD Blocker A
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RT
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5 bottle (250 mL)
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MSD Phosphate Buffer 5X
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RT
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5 bottle (50 mL)
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Product Inserts
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Product Highlights
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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Biomarkers of Tuberculosis Severity and Treatment Effect: A Directed Screen of 70 Host Markers in a Randomized Clinical Trial
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A multi-biomarker score measures rheumatoid arthritis disease activity in the BeSt study.
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A phase II and biomarker study of ramucirumab, a human monoclonal antibody targeting the VEGF receptor-2 in patients with advanced hepatocellular cancer.
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Efficacy and safety of mavrilimumab in subjects with rheumatoid arthritis.
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