V-PLEX Human IL-1RA Kit Overview
The V-PLEX Human IL-1RA Kit is optimized for long-term and multi-site biomarker studies that require reliable, reproducible results with lot-to-lot consistency. The V-PLEX Human IL-1RA Kit enables the sensitive detection of native IL-1RA in multiple sample types including serum, plasma, urine, cell culture supernatants, and PBMCs. To learn more about the advantages of V-PLEX assays
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The V-PLEX Human IL-1RA assay is also available in a multiplex combination with other related cytokines in the V-PLEX Cytokine Panel 2 (human) Kit (catalog # K15084G, K15084D).
V-PLEX Human IL-1RA Assay Features
- Validated to measure human IL-1RA in serum, plasma, cell culture supernatants, PBMCs, and urine
- Dynamic range: 1.12 pg/mL – 650 pg/mL
- LLOQ: 9.19 pg/mL
Typical Data
Calibration curve accuracy and precision for the V-PLEX Human IL-1RA assay were assessed and representative data are presented below.
Figure 1. V-PLEX Human IL-1RA Calibration Curve
Figure 2. V-PLEX Human IL-1RA Data
IL-1RA Analyte
Description - Human interleukin 1 receptor antagonist (IL1RN, IL1F3, IL1RA, IRAP; Gene ID: 3557) is a protein that is produced in at least two forms, one that stays inside the cell and one that is secreted. This assay is for the secreted version. IL-1RA is secreted by various cell types including epithelial cells, monocytes, and adipocytes. It binds to the IL-1 receptor thus preventing signaling by both IL-1α and IL 1β cytokines.
Disease Research Focus - Asthma and various autoimmune disorders.
Application Area - Inflammation, cytokines, and chemokines
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Specifications
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Kit Contents
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Documentation
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-1RA
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Goat Polyclonal
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Dynamic Range
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1.12 - 650 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant, Urine
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Recombinant standards
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Expressed in E. coli
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LLOD (Sensitivity)
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1.12 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 vial
(75 μL)
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Human IL-1RA SECTOR Plate
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2-8°C
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1 ea
(1 ea)
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Diluent Assembly 3 (includes Diluent 3 - 5 mL, and Diluent 43 - 10 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 vial
(1 vial)
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2-8°C
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1 vial
(375 μL)
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Human IL-1RA SECTOR Plate
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2-8°C
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5 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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25 vial
(1 vial)
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2-8°C
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5 vial
(375 μL)
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Human IL-1RA SECTOR Plate
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2-8°C
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25 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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5 ea
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RT
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5 ea
(50 mL)
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Product Inserts
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Product Highlights
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Certificate of analysis
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SDS
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters