V-PLEX Human IL-17A/F Kit Overview
The V-PLEX Human IL-17A/F Kit is optimized for long-term and multi-site biomarker studies that require reliable, reproducible results with lot-to-lot consistency. The V-PLEX Human IL-17A/F Kit enables the sensitive detection of native IL17A/F in multiple sample types including serum, plasma, urine, cell culture supernatants, and PBMCs. To learn more about the advantages of V-PLEX assays
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The V-PLEX Human IL-17A/F assay is available as an individual assay kit as well as in a multiplex combination with other related cytokines in the V-PLEX Cytokine Panel 2 (human) Kit (catalog # K15084G, K15084D).
V-PLEX Human IL-17A/F Assay Features
- Validated to measure human IL-17A/F in serum, plasma, cell culture supernatants, PBMCs, and urine
- Dynamic range: 0.930 pg/mL – 3,900 pg/mL
- LLOQ: 7.57 pg/mL
Typical Data
Calibration curve accuracy and precision for the V-PLEX Human IL-17A/F Assay were assessed and representative data are presented below.
Figure 1. V-PLEX Human IL-17A/F Calibration Curve
Figure 2. V-PLEX Human IL-17A/F Data
IL-17A/F Analyte
Description - Human IL-17A/F [Gene ID: 3605 (IL-17A), 112744 (IL-17F)] is a cross-linked heterodimer composed of IL-17A (CTLA8) and IL-17F proteins that is secreted by IL-23 activated TH17 cells. IL-17A and IL-17F are both prototypical of the IL-17 family of cytokines. IL-17A/F binds to IL-17RA and recruits neutrophils with intermediate potency between IL-17A and IL-17F homodimers.
Disease Research Area - Autoimmune and inflammatory diseases including asthma, rheumatoid arthritis, and inflammatory bowel disease (IBD).
Application Area - Inflammation, cytokines, and chemokines
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Specifications
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Kit Contents
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Documentation
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-17A/F
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Mouse Recombinant
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Detection Antibody
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Mouse Monoclonal
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Dynamic Range
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0.930 – 3,900 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant, Urine
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Recombinant standards
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Expressed in CHO cells
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LLOD (Sensitivity)
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0.930 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 vial
(75 μL)
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Human IL-17A/F SECTOR Plate
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2-8°C
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1 ea
(1 ea)
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Diluent Assembly 3 (includes Diluent 3 - 5 mL, and Diluent 43 - 10 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 vial
(1 vial)
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2-8°C
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1 vial
(375 μL)
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Human IL-17A/F SECTOR Plate
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2-8°C
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5 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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1 ea
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RT
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1 ea
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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25 vial
(1 vial)
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2-8°C
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5 vial
(375 μL)
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Human IL-17A/F SECTOR Plate
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2-8°C
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25 ea
(1 ea)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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5 ea
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RT
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5 ea
(50 mL)
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Product Inserts
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Product Highlights
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SDS
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Order Details
Catalog No
Size
Price in USD
Qty
Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters