Chemokines are small chemotactic cytokines with molecular weights around 8–10 kDa that are capable of inducing directed chemotaxis. Four cysteine residues in conserved locations result in a compact 3-dimensional structure. Based on the spacing of the first two cysteine residues, chemokines are divided into four families: CC chemokines, CXC chemokines, C chemokines, and CX3C chemokines, where C represents cysteine and X represents any other amino acid. Chemokines function by activating specific G protein-coupled receptors resulting in migration of inflammatory and non-inflammatory cells. The pro-inflammatory chemokines are responsible for migration of immune cells to the infection site, while the homeostatic chemokines are responsible for recruiting cells for tissue maintenance and development. The Chemokine Panel 1 (NHP) Kit provides assay-specific components for the quantitative determination of seven CC chemokine (MIP-1β, eotaxin-3, TARC, MIP-1α, MCP-1, MDC, MCP-4) and two CXC chemokine assays (IP-10, IL-8). This kit may be used for the analysis of all or any combination of these chemokine biomarkers in cell culture supernatants, serum, plasma, and urine.
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Specifications
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Kit Contents
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References
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Documentation
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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Eotaxin-3, IL-8, IL-8 (HA), IP-10, MCP-1, MCP-4, MDC, MIP-1α, MIP-1β, TARC
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Species
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Non-human primate
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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MIP-1β: Mouse Monoclonal
Eotaxin-3: Mouse Monoclonal
TARC: Mouse Monoclonal
IP-10: Mouse Monoclonal
MIP-1α: Mouse Monoclonal
IL-8(HA): Mouse Monoclonal
MCP-1: Mouse Monoclonal
MDC: Mouse Monoclonal
MCP-4: Mouse Monoclonal
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Detection Antibody
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MIP-1β: Mouse Monoclonal
Eotaxin-3: Mouse Monoclonal
TARC: Mouse Monoclonal
IP-10: Mouse Monoclonal
MIP-1α: Mouse Monoclonal
IL-8(HA): Goat Polyclonal
MCP-1: Mouse Monoclonal
MDC: Mouse Monoclonal
MCP-4: Mouse Monoclonal
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Dynamic Range
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MIP-1β: 0.17–750 pg/mL
Eotaxin-3: 1.77–3750 pg/mL
TARC: 0.22–1120 pg/mL
IP-10: 0.37–500 pg/mL
MIP-1α: 3.02–743 pg/mL
IL-8(HA): 95.6–43 400 pg/mL
MCP-1: 0.09–375 pg/mL
MDC: 1.22–7500 pg/mL
MCP-4: 1.69–469 pg/mL
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Sample Type
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Serum, Plasma, Urine, Cell Culture Supernatant
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Recombinant standards
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MIP-1β: Expressed in E. coli
Eotaxin-3: Expressed in E. coli
TARC: Expressed in E. coli
IP-10: Expressed in E. coli
MIP-1α: Expressed in E. coli
IL-8(HA): Expressed in E. coli
MCP-1: Expressed in E. coli
MDC: Expressed in E. coli
MCP-4: Expressed in E. coli
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LLOD (Sensitivity)
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MIP-1β: 0.17 pg/mL
Eotaxin-3: 1.77 pg/mL
TARC: 0.22 pg/mL
IP-10: 0.37 pg/mL
MIP-1α: 3.02 pg/mL
IL-8(HA): 95.6 pg/mL
MCP-1: 0.09 pg/mL
MDC: 1.22 pg/mL
MCP-4: 1.69 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 vial
(75 μL)
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Diluent Assembly 3 (includes Diluent 3 - 5 mL, and Diluent 43 - 10 mL)
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≤-10°C
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1 each
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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2-8°C
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1 vial
(75 μL)
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Chemokine Panel 1 (human) Plate
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2-8°C
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1 each
(1 each)
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RT
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1 each
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 vial
(1 vial)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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2-8°C
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1 vial
(375 μL)
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Chemokine Panel 1 (human) Plate
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2-8°C
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5 each
(1 each)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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1 each
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RT
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1 each
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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25 vial
(1 vial)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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2-8°C
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5 vial
(375 μL)
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Chemokine Panel 1 (human) Plate
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2-8°C
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25 each
(1 each)
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Diluent Assembly 4 (includes Diluent 3 - 25 mL, and Diluent 43 - 50 mL)
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≤-10°C
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5 each
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RT
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5 each
(50 mL)
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Title
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Journal
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Year
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Protection Against Lethal Multidrug-Resistant Bacterial Infections Using Macrophage Cell Therapy
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CCL11 is increased in the CNS in chronic traumatic encephalopathy but not in Alzheimer’s disease
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Cytokine biomarkers in tear film for primary open-angle glaucoma
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Kinetics and Biomarkers of Severe Cytokine Release Syndrome after CD19 Chimeric Antigen Receptor-modified T Cell Therapy.
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A Pilot Study of Preoperative Single-Dose Ipilimumab and/or Cryoablation in Women with Early-Stage Breast Cancer with Comprehensive Immune Profiling.
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View All
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Product Inserts
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Product Highlights
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Certificate of analysis
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View More
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SDS
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Kit Layout (Add-in)
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Order Details
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Size
Price in USD
Qty
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Scientific/Technical Support
Instrument Support
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