U-PLEX NHP IL-12p70 Assay Overview
The U-PLEX NHP IL-12p70 Assay maximizes your flexibility to design an experiment that measures IL-12p70 alone or multiplexed with MSD U-PLEX assays, your own antibody pairs, or a combination of both. U-PLEX assays demonstrate high sensitivity, excellent precision, and up to 5-logs of linear dynamic range. Plates are read in less than 90 seconds on any MSD instrument, using no complicated fluidics or calibration procedures.
U-PLEX NHP IL-12p70 Assay Features
- Simple protocol similar to ELISAs, yet uses less sample
- Measures non-human primate (NHP) IL-12p70 in serum, plasma, and cell culture supernatants
- Create custom multiplexes using a selection of U-PLEX assays, your own antibody pairs or both
Assay Format
- Singleplex – includes MSD GOLD Small Spot Streptavidin Plates
- Multiplex – includes U-PLEX multiplex plates
- Both formats use the same assay components for efficient transfer between single- and multiplex configurations.
IL-12p70 Analyte
Description - IL-12p70 (or IL-12) is a disulfide-linked heterodimer consisting of p35 and p40 subunits that is mainly produced by macrophages, neutrophils, and B cells. IL-12, IL-23, IL-27, and IL-35 are all heterodimeric proteins and members of the IL-12 cytokine family. IL-12p70 activates T cells and NK cells to produce IFN-γ.
Disease Focus - Peritonitis, Crohn’s disease, viral infections, and chronic toxoplasmosis.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-12p70
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Species
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Non-human primate
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Mouse Monoclonal
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Dynamic Range
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0.54-5,300 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant
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Recombinant standards
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Expressed in Sf21 insect cells
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LLOD (Sensitivity)
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0.54 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each
(1 plate size)
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2-8°C
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1 vial
(1 vial)
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2-8°C
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1 each
(1 plate)
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2-8°C
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1 bottle
(50 mL)
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≤-10°C
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1 bottle
(10 mL)
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≤-10°C
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1 bottle
(8 mL)
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RT
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1 each
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each
(5 plate size)
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2-8°C
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5 vial
(1 vial)
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2-8°C
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5 each
(1 plate)
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2-8°C
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1 bottle
(50 mL)
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≤-10°C
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1 bottle
(50 mL)
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≤-10°C
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1 bottle
(40 mL)
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RT
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1 each
(50 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 each
(5 plate size)
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2-8°C
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25 vial
(1 vial)
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2-8°C
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25 each
(1 plate)
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2-8°C
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5 bottle
(50 mL)
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≤-10°C
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5 bottle
(50 mL)
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≤-10°C
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5 bottle
(40 mL)
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RT
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5 each
(50 mL)
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Product Inserts
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Datasheets
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SDS
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Handouts
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Title
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Journal
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Year
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Cytokine biomarkers in tear film for primary open-angle glaucoma
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A Pilot Study of Preoperative Single-Dose Ipilimumab and/or Cryoablation in Women with Early-Stage Breast Cancer with Comprehensive Immune Profiling.
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Effect of Wild-Type Shigella Species and Attenuated Shigella Vaccine Candidates on Small Intestinal Barrier Function, Antigen Trafficking, and Cytokine Release.
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Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome.
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Abdominal obesity, independent from caloric intake, accounts for the development of intestinal tumors in Apc1638N/+ female mice.
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View All
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