U-PLEX NHP IL-12p70 Assay Overview
The U-PLEX NHP IL-12p70 Assay maximizes your flexibility to design an experiment that measures IL-12p70 alone or multiplexed with MSD U-PLEX assays, your own antibody pairs, or a combination of both. U-PLEX assays demonstrate high sensitivity, excellent precision, and up to 5-logs of linear dynamic range. Plates are read in less than 90 seconds on any MSD instrument, using no complicated fluidics or calibration procedures.

U-PLEX NHP IL-12p70 Assay Features

• Simple protocol similar to ELISAs, yet uses less sample
• Measures non-human primate (NHP) IL-12p70 in serum, plasma, and cell culture supernatants
• Create custom multiplexes using a selection of U-PLEX assays, your own antibody pairs or both

Assay Format

• Singleplex – includes MSD GOLD Small Spot Streptavidin Plates
• Multiplex – includes U-PLEX multiplex plates
• Both formats use the same assay components for efficient transfer between single- and multiplex configurations.

IL-12p70 Analyte
Description - IL-12p70 (or IL-12) is a disulfide-linked heterodimer consisting of p35 and p40 subunits that is mainly produced by macrophages, neutrophils, and B cells. IL-12, IL-23, IL-27, and IL-35 are all heterodimeric proteins and members of the IL-12 cytokine family. IL-12p70 activates T cells and NK cells to produce IFN-γ.

Disease Focus - Peritonitis, Crohn’s disease, viral infections, and chronic toxoplasmosis.