U-PLEX Mouse TNF-α Assay


MSD offers a range of individual assays utilizing U-PLEX Antibody Sets that provide a rapid and convenient method for measuring biomarkers in complex matrices. The individual assays are offered on MSD GOLD Small Spot Streptavidin Plates and use the same antibody sets and diluents as the U-PLEX multiplex assays. This allows for efficient transfer between the individual assay and a higher throughput multiplex configuration. Typical of assays developed on the MSD platform, the individual assays have high sensitivity, excellent precision, provide up to five-logs of linear dynamic range, and require minimal sample volume. Mouse tumor necrosis factor alpha (TNF-α), also known as tumor necrosis factor ligand superfamily member 2 (TNFSF2) and cachectin, is a 25.9 kDa cytokine. TNF is a transmembrane protein that oligomerizes intracellularly to form a non-covalent homotrimer. The membrane-bound soluble portion of the homotrimer is cleaved by TACE/ADAM17 to form TNF-α. The homotrimer binds to the receptors TNF RI and TNF RII, both of which are also expressed as homotrimers. TNF-α is mainly secreted by macrophages and can induce apoptosis in some tumor cell lines. It stimulates IL-1, which induces cachexia and causes fever. The intracellular form of TNF induces IL-12 production in dendritic cells. It can induce sepsis and inflammation and can inhibit tumorigenesis and viral replication. The U-PLEX Mouse TNF-α Assay provides the components for the quantitative determination of both natural and recombinant TNF-α in serum, plasma, and cell culture supernatants.

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1 plate
5 plates
25 plates
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