MSD offers a range of individual assays utilizing U-PLEX Antibody Sets that provide a rapid and convenient method for measuring biomarkers in complex matrices. The individual assays are offered on MSD GOLD Small Spot Streptavidin Plates and use the same antibody sets and diluents as the U-PLEX multiplex assays. This allows for efficient transfer between the individual assay and a higher throughput multiplex configuration. Typical of assays developed on the MSD platform, the individual assays have high sensitivity, excellent precision, provide up to five-logs of linear dynamic range, and require minimal sample volume.
Mouse interleukin-12p70 (IL-12p70) is a disulfide-linked heterodimer consisting of IL-12a (p35 subunit) and IL-12b (p40 subunit) that is mainly produced by macrophages and T lymphocytes. The IL-12a subunit also dimerizes with Ebi3 to form IL-35, and the IL-12b subunit dimerizes with IL-23a to form IL-23. IL-12, IL-23, IL-27, and IL-35 are all heterodimeric proteins and members of IL-12 cytokine family. IL-12p70 activates T cells and natural killer cells that stimulate the production of IFN-γ. It is involved in a number of disorders including peritonitis and chronic toxoplasmosis. The U-PLEX Mouse IL-12p70 Assay provides the components for the quantitative determination of both natural and recombinant IL-12p70 in serum, plasma, and cell culture supernatants.
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Specifications
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Kit Contents
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Documentation
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References
Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IL-12p70
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Species
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Mouse
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Rat Monoclonal
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Detection Antibody
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Rat Monoclonal
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LLOD (Sensitivity)
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48 pg/mL
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Dynamic Range
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48-89,000 pg/mL
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Sample Type
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Serum, Plasma, Cell Culture Supernatant
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Recombinant standards
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Expressed in Sf21 insect cells
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each (1 plate size)
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2-8°C
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1 vial (1 vial)
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2-8°C
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1 each (1 plate)
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2-8°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (10 mL)
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≤-10°C
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1 bottle (8 mL)
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RT
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1 bottle (18 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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1 each (5 plate size)
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2-8°C
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5 vial (1 vial)
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2-8°C
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5 each (1 plate)
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2-8°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (50 mL)
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≤-10°C
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1 bottle (40 mL)
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RT
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1 bottle (90 mL)
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Product Description
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Storage
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Quantity per Kit
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2-8°C
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5 each (5 plate size)
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2-8°C
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25 vial (1 vial)
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2-8°C
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25 each (1 plate)
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2-8°C
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5 bottle (50 mL)
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≤-10°C
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5 bottle (50 mL)
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≤-10°C
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5 bottle (40 mL)
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RT
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5 bottle (90 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics
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Cytokine biomarkers in tear film for primary open-angle glaucoma
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A Pilot Study of Preoperative Single-Dose Ipilimumab and/or Cryoablation in Women with Early-Stage Breast Cancer with Comprehensive Immune Profiling.
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Effect of Wild-Type Shigella Species and Attenuated Shigella Vaccine Candidates on Small Intestinal Barrier Function, Antigen Trafficking, and Cytokine Release.
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Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome.
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View All
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