MSD offers a range of individual assays utilizing U-PLEX Antibody Sets that provide a rapid and convenient method for measuring biomarkers in complex matrices. The individual assays are offered on MSD GOLD Small Spot Streptavidin Plates and use the same antibody sets and diluents as the U-PLEX multiplex assays. This allows for efficient transfer between the individual assay and a higher throughput multiplex configuration. Typical of assays developed on the MSD platform, the individual assays have high sensitivity, excellent precision, provide up to five-logs of linear dynamic range, and require minimal sample volume. Mouse interleukin-12p70 (IL-12p70) is a disulfide-linked heterodimer consisting of IL-12a (p35 subunit) and IL-12b (p40 subunit) that is mainly produced by macrophages and T lymphocytes. The IL-12a subunit also dimerizes with Ebi3 to form IL-35, and the IL-12b subunit dimerizes with IL-23a to form IL-23. IL-12, IL-23, IL-27, and IL-35 are all heterodimeric proteins and members of IL-12 cytokine family. IL-12p70 activates T cells and natural killer cells that stimulate the production of IFN-γ. It is involved in a number of disorders including peritonitis and chronic toxoplasmosis. The U-PLEX Mouse IL-12p70 Assay provides the components for the quantitative determination of both natural and recombinant IL-12p70 in serum, plasma, and cell culture supernatants.