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U-PLEX Human TGF-β1 Assay

U-PLEX Human TGF-β1 Assay Overview
The U-PLEX Human TGF-β1 Assay maximizes your flexibility to design an experiment that measures TGF-β1 alone or multiplexed with other MSD U-PLEX assays, your own antibody pairs, or a combination of both. This assay accurately quantitates native and recombinant human TGF-β1 in multiple sample types. To learn more about the advantages of U-PLEX assays click here.

U-PLEX Human TGF-β1 Assay Features

Measures human TGF-β1 in serum, plasma, and cell culture supernatants
Dynamic range: 9.1 pg/mL – 37,000 pg/mL
LLOD: 9.1 pg/mL

Typical Data
Calibration curve accuracy and precision for the U-PLEX Human TGF-β1 assay were assessed and representative data are presented below.

Figure 1. U-PLEX Human TGF-β1 Calibration Curve

Figure 2. U-PLEX Human TGF-β1 Data

TGF-β1 Analyte

Description - Human transforming growth factor β1 (TGFbeta, TGFB1, CED, DPD1; Gene ID: 7040) is a member of at least three ligands in the TGF family that are highly pleiotropic and potent cytokines. They are secreted by almost all cell types, and as such their activity is tightly controlled. TGFs are secreted and complexed with two other proteins, LAP and TGFBP, and require proteolytic cleavage to be active. TGFs are highly conserved. TGFs regulate T cell maturation and differentiation and inhibit B cell proliferation.

Disease Focus/Application Area - Cancer, Marfan syndrome, kidney disease, diabetes, and multiple sclerosis.

Specifications
Kit Contents
References
Resources

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TGF-β1
Species		Human
Instrument		MESO QuickPlex SQ 120 , SECTOR Imager 2400 , SECTOR Imager 6000 , SECTOR S 600
Plate Type		96-well
Capture Antibody		Mouse Monoclonal
Detection Antibody		Chicken Polyclonal
Dynamic Range		9.1 - 37,000 pg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
LLOD (Sensitivity)		9.1 pg/mL
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component

Product Description	Storage	Quantity per Kit
U-PLEX TGF-β1 Antibody Set (1 Plate Size) Capture antibody: 1X, Detection antibody: 100X	2-8°C	1 ea (1 plate size)
Calibrator 11	2-8°C	1 vial (NA vial)
MSD GOLD 96-Well Small Spot Streptavidin SECTOR Plate	2-8°C	1 ea (1 ea)
Diluent 100	2-8°C	1 ea (50 mL)
Diluent 43	≤-10°C	1 Bottle (10 mL)
Diluent 3, 8 mL	≤-10°C	1 ea (8 mL)
Read Buffer T 4X	RT	1 ea (50 mL)

Product Description	Storage	Quantity per Kit
U-PLEX TGF-β1 Antibody Set (5 Plate Size) Capture antibody: 1X, Detection antibody: 100X	2-8°C	1 ea (5 plate size)
Calibrator 11	2-8°C	5 vial (NA vial)
MSD GOLD 96-Well Small Spot Streptavidin SECTOR Plate	2-8°C	5 ea (1 ea)
Diluent 100	2-8°C	1 ea (50 mL)
Diluent 43	≤-10°C	1 Bottle (50 mL)
Diluent 3, 40 mL	≤-10°C	1 ea (40 mL)
Read Buffer T 4X	RT	1 ea (50 mL)

Product Description	Storage	Quantity per Kit
U-PLEX TGF-β1 Antibody Set (5 Plate Size) Capture antibody: 1X, Detection antibody: 100X	2-8°C	5 ea (5 plate size)
Calibrator 11	2-8°C	25 vial (NA vial)
MSD GOLD 96-Well Small Spot Streptavidin SECTOR Plate	2-8°C	25 ea (1 ea)
Diluent 100	2-8°C	5 ea (50 mL)
Diluent 43	≤-10°C	5 Bottle (50 mL)
Diluent 3, 40 mL	≤-10°C	5 ea (40 mL)
Read Buffer T 4X	RT	5 ea (50 mL)

Order Details

Catalog No

Size

Price in USD

Qty

K151XWK-1

1 plate

$335.00

Usually ships in 1-3 days

K151XWK-2

5 plates

$1,442.00

Usually ships in 1-3 days

K151XWK-4

25 plates

$6,438.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Title	Journal	Year
Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome. Meissner EG, Wu D, Osinusi A, Bon D, Virtaneva K, Sturdevant D, Porcella S, Wang H, Herrmann E, McHutchison J, Suffredini AF, Polis M, Hewitt S, Prokunina-Olsson L, Masur H, Fauci AS, Kottilil S.	J Clin Invest	2014

Product Inserts		U-PLEX Biomarker Group 2 - Multiplex - Product Insert U-PLEX Biomarker Group 2 - Singleplex - Product Insert
Product Highlights		U-PLEX Platform Highlights
SDS		Biotin-conjugated Antibodies Safety Data Sheet - English Diluent 100 and Diluent 101 - English Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 - English Diluent 41, Diluent 43 and Diluent 45 - English Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) - English
Datasheets		DS U-PLEX Human TGF-β1

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U-PLEX Human TGF-β1 Assay

Kit Contents for Catalog No. K151XWK-1

Kit Contents for Catalog No. K151XWK-2

Kit Contents for Catalog No. K151XWK-4

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