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S-PLEX Human IL-5 Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-5 Assay has the essential components for an ultrasensitive assay that measures IL-5 in a single-analyte format. For detailed instructions on running the IL-5 assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
IL5, EDF, TRF, interleukin 5, allergic rhinitis, pneumonia

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-5
Species		Human
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		2 fg/mL
Dynamic Range		2 - 32,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Recombinant standards		Expressed in Insect cell line
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3567
UniProt ID(s)		P05113
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151J3S-1

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-5 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 23 (includes Diluent 43 – 10 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151J3S-2

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-5 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151J3S-4

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-5 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Kit Contents for Catalog No. K151J3S-21

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-5 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 23 (includes Diluent 43 – 10 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151J3S-22

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-5 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151J3S-24

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-5 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-5 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 24 (includes Diluent 43 – 50 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

K151J3S

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K151J3S-1

1 plate size

$882.00

Usually ships in 1-3 days

K151J3S-2

5 plate size

$3,735.00

Usually ships in 1-3 days

K151J3S-4

25 plate size

$15,873.00

Usually ships in 1-3 days

K151J3S-21

1 each

$882.00

Usually ships in 1-3 days

K151J3S-22

5 each

$3,735.00

Usually ships in 1-3 days

K151J3S-24

25 each

$15,873.00

Usually ships in 1-3 days

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Product Inserts		S-PLEX IL-5 Kit Product Insert
Certificate of analysis		K00S0108 with Extension K00S0166 K00S0141 K00S0073 K00S0118 K00S0053 K00S0026 with Extension K00S0026 K00S0008A
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 41, Diluent 43 and Diluent 45 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer A

Title	Journal	Year
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Early and late effects of maternal experience on hippocampal neurogenesis, microglia, and the circulating cytokine milieu Rand S. Eid, Jessica A. Chaiton, Stephanie E. Lieblich, Tamara S. Bodnar, Joanne Weinberg, Liisa A.M. Galea	Neurobiology of Aging	2019
Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy. Koppes SA, Brans R, Ljubojevic Hadzavdic S, Frings-Dresen MH, Rustemeyer T, Kezic S.	Int Arch Allergy Immunol.	2016
A salmon peptide diet alleviates experimental colitis as compared with fish oil Grimstad T, Bjorndal B, Cacabelos D, Aasprong OG, Omdal R, Svardal A, Bohov P, Pamplona R, Portero-Otin M, Berge RK, Hausken T.	Journal of Nutritional Science	2013
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S-PLEX Human IL-5 Kit

Kit Contents for Catalog No. K151J3S-1

Kit Contents for Catalog No. K151J3S-2

Kit Contents for Catalog No. K151J3S-4

Kit Contents for Catalog No. K151J3S-21

Kit Contents for Catalog No. K151J3S-22

Kit Contents for Catalog No. K151J3S-24

Order Details

K151J3S

S 600

SQ 120

Q 60

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