R-PLEX Rat GM-CSF Antibody Set Overview The R-PLEX Rat GM-CSF Antibody Set includes a matched capture and detection antibody pair and calibrator for the quick and easy measurement of GM-CSF in biological samples. R-PLEX products leverage MSD’s electrochemiluminescence technology for better sensitivity and a larger dynamic range than traditional ELISAs. R-PLEX matched antibody sets enable the development of singleplex and multiplex immunoassays. Individual assays may be developed using MSD avidin- or streptavidin-coated plates. Custom multiplex assays with any combination of R-PLEX analytes can be developed on U-PLEX MULTI-SPOT plates. To learn more about the advantages of R-PLEX products
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R-PLEX Rat GM-CSF Antibody Set Features - Measures rat GM-CSF in Serum, Plasma
- Dynamic range: 2.2 - 100,000 pg/mL
- Median LLOD: 2.2 pg/mL
- Can be used to develop individual analyte assays as well as multiplex panels
Typical Data Calibration curve for the R-PLEX Rat GM-CSF Antibody Set was assessed and representative data is presented below.
Figure 1. R-PLEX Rat GM-CSF Antibody Set Calibration Curve 
GM-CSF Analyte
Description - Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF, CSF2) is pivotal in regulating the functions of granulocyte and macrophage lineage populations at all stages of growth and maturation. GM-CSF is secreted by a variety of cell types including T and B cells, macrophages, NK cells, mast cells, fibroblasts, endothelial cells, and adipocytes. As a key player in immune and inflammatory mechanisms, GM-CSF stimulates Th1 biased immune response, angiogenesis, allergic inflammation, and autoimmunity development.
Application Area - Autoimmune and Inflammation, Oncology
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Recommended Components
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Specifications
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Kit Contents
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References
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Documentation
You will need plates, diluents, and read buffer to run your assay. Please select from the below menu.
R-PLEX Sets are intended to be used with other MSD components such as plates, diluents and MSD Read Buffer. If you don’t already have these items, you can purchase them along with your Antibody Set(s). The “Recommended Components” tab provides helpful suggestions or you can call MSD Customer Service (Phone number) for assistance. R-PLEX Sets can also be used with other antibody sets such as U-PLEX antibody sets (make a link to UPLEX landing page) for multiplex measurements.
Catalog No.
Product Name
Size
R50AA-4
R92TG-3
L45SA-1
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Application(s)
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Immunology/Inflammation
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Oncology & Cancer
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Analyte(s)
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GM-CSF
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Species
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Rat
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Goat Polyclonal
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Dynamic Range
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2.15-100,000 pg/mL
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Sample Type
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Serum, Plasma
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Recombinant standards
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Recombinant Expressed in E.coli
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LLOD (Sensitivity)
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2.15 pg/mL
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Gene ID(s)
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116630
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UniProt ID(s)
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P48750
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Assay Diluent(s)
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Diluent 42
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Antibody Diluent(s)
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Diluent 40
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Product Description
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Storage
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Quantity per Kit
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Rat GM-CSF Antibody Set (5 Plate Size)
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2-8°C
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1 ea
(5 plate size)
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Rat GM-CSF Calibrator
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≤-70°C
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5 vial
(1 vial)
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Product Description
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Storage
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Quantity per Kit
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Rat GM-CSF Antibody Set (5 Plate Size)
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2-8°C
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10 ea
(5 plate size)
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Rat GM-CSF Calibrator
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≤-70°C
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50 vial
(1 vial)
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Title
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Journal
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Year
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Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy.
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Endogenous intrahepatic IFNs and association with IFN-free HCV treatment outcome.
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Association of IL-12p70 and IL-6:IL-10 ratio with autism-related behaviors in 22q11.2 deletion syndrome: A preliminary report.
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Effect of short-term pair housing of juvenile Rhesus Macaques (Macaca mulatta) on immunologic parameters.
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Experimental pain and opioid analgesia in volunteers at high risk for obstructive sleep apnea
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Product Inserts
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Product Highlights
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SDS
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Handouts
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Datasheets
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