The Phospho(Ser15)/Total p53 Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-p53 (Ser15) and total p53 in human whole cell lysates.
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Specifications
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Kit Contents
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References
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Documentation
Application(s)
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Phosphoproteins
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Oncology & Cancer
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Ubiquitinated Proteins
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Intracellular Signaling
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Analyte(s)
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p53
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Phospho-p53: Mouse Monoclonal Total p53: Mouse Monoclonal
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Detection Antibody
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Phospho-p53: Goat Polyclonal Total p53: Goat Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8°C
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1 each (0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8°C
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1 each (1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8°C
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1 each (1 each)
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SULFO-TAG Anti-Total p53 Antibody
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2-8°C
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1 vial (75 μL)
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Phospho(Ser15)/Total p53 Plate
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2-8°C
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1 each (1 each)
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2-8°C
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1 each (50 mL)
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2-8°C
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1 each (200 mL)
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RT
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1 each (50 mL)
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RT
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1 each (15 g)
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RT
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1 each (1 g)
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MSD Blocker D-R, 0.05 mL
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≤-10°C
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1 each (1 each)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8°C
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1 each (1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8°C
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1 each (1 each)
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Protease Inhibitor Solution 0.5mL
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2-8°C
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1 each (1 each)
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SULFO-TAG Anti-Total p53 Antibody
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2-8°C
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1 vial (375 μL)
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Phospho(Ser15)/Total p53 Plate
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2-8°C
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5 each (1 each)
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2-8°C
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1 each (50 mL)
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2-8°C
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1 each (200 mL)
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RT
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1 each (50 mL)
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RT
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1 each (15 g)
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RT
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1 each (1 g)
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MSD Blocker D-R
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≤-10°C
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1 each (0.2 mL)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8°C
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1 each (1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8°C
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1 each (1 each)
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Protease Inhibitor Solution 2.0mL
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2-8°C
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1 each (1 each)
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SULFO-TAG Anti-Total p53 Antibody
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2-8°C
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4 vial (375 μL)
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Phospho(Ser15)/Total p53 Plate
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2-8°C
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20 each (1 each)
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2-8°C
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1 each (200 mL)
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2-8°C
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1 each (1000 mL)
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RT
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1 each (200 mL)
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RT
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1 each (15 g)
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RT
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1 each (2 g)
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MSD Blocker D-R
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≤-10°C
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4 each (0.2 mL)
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Title
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Journal
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Year
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Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
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Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen.
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Multiplexing siRNAs to compress RNAi-based screen size in human cells.
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Product Inserts
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Datasheets
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SDS
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Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters