Phospho-Histone H3 (Ser 10) Base Kit

Histone H3 is one of the five main classes of histones, which are found in the nucleus of eukaryotic cells, and are the main building blocks of chromatin. It has an amino terminal tail and post-translational modification of this tail is involved in chromatin relaxation and transcriptional regulation. Such epigenetic modifications of histones have been shown to play a role in cancer, embryonic stem cell function, and mammalian development. This kit provides basic components for the quantitative determination of phospho-Histone H3 (Ser10) in human, mouse, and rat whole cell lysates. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.

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