This kit provides assay-specific components for the quantitative determination of phosphorlated BCL2-antagonist of cell death (phospho-BAD) (Ser112) in human and non-human primate whole cell lysates. Phosphorylation of BAD by kinases such as Akt, PKA, and p90RSK blocks its pro-death activity. phospho-BAD binds to the 14-3-3 protein and displaces its binding to Bcl-2 and Bcl-xL.
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Specifications
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Kit Contents
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References
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Documentation
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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BAD
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Species
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Human, Non-human primate
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Instrument
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MESO QuickPlex SQ 120
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Rabbit Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8°C
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8°C
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1 each
(0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8°C
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1 each
(1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8°C
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1 each
(1 each)
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SULFO-TAG Anti-BAD Antibody
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2-8°C
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1 vial
(75 μL)
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Phospho-BAD Plate
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2-8°C
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1 each
(1 each)
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2-8°C
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1 each
(50 mL)
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2-8°C
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1 each
(200 mL)
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RT
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1 each
(50 mL)
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RT
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1 each
(15 g)
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RT
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1 each
(1 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8°C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8°C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8°C
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1 each
(1 each)
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SULFO-TAG Anti-BAD Antibody
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2-8°C
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1 vial
(375 μL)
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Phospho-BAD Plate
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2-8°C
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5 each
(1 each)
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2-8°C
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1 each
(50 mL)
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2-8°C
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1 each
(200 mL)
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RT
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1 each
(50 mL)
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RT
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1 each
(15 g)
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RT
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1 each
(1 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8°C
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1 each
(1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8°C
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1 each
(1 each)
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Protease Inhibitor Solution 2.0mL
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2-8°C
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1 each
(1 each)
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SULFO-TAG Anti-BAD Antibody
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2-8°C
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4 vial
(375 μL)
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Phospho-BAD Plate
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2-8°C
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20 each
(1 each)
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2-8°C
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1 each
(200 mL)
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2-8°C
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1 each
(1000 mL)
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RT
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1 each
(200 mL)
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RT
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1 each
(15 g)
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RT
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1 each
(2 g)
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Title
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Journal
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Year
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Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
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A novel peptide, colivelin, prevents alcohol-induced apoptosis in fetal brain of C57BL/6 mice: Signaling pathway investigations.
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Multiplexing siRNAs to compress RNAi-based screen size in human cells.
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Product Inserts
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Datasheets
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SDS
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Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters