Poly (ADP-ribose) polymerase (PARP) is a nuclear enzyme activated by DNA damage and was one of the first identified death subsrates for caspase-3 in apoptosis. Active PARP transfers ADP-ribose from NAD+ to nuclear proteins, and this modification has been implicated in many fundamental processes like DNA repair, chromatin stability, cell proliferation, and cell death. PARP becomes inactive following cleavage, however, the small cleavage product does retain its DNA-binding capabilities, blocking access to damaged DNA and inhibiting further DNA repair. This kit provides basic components for the quantitative determination of PARP (cleaved) (Asp214) in human whole cell lysates. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components.) Base kits are most commonly used for high-throughput screening and assay development.