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The Mouse/Rat Hypoxia Base Kit provides basic components for the simultaneous quantitative determination of both natural and recombinant EPO and VEGF in mouse and rat tissue culture supernatant. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.
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Specifications
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Documentation
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References
Application(s)
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Hypoxia
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Cardiovascular
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Analyte(s)
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EPO, VEGF-A
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Species
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Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO QuickPlex SQ 120
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MESO QuickPlex SQ 120MM
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MESO SECTOR S 600
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MESO SECTOR S 600MM
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Plate Type
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96-well
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Capture Antibody
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EPO: Mouse monoclonal VEGF: Goat Polyclonal
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Detection Antibody
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EPO: Mouse monoclonal VEGF: Goat Polyclonal
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LLOD (Sensitivity)
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Depending on the desired application, the protocol can be applied to mouse or rat calibrators. Mouse EPO: 2.3 pg/mL Rat EPO: 2.0 pg/mL Mouse VEGF: 1.8 pg/mL Rat VEGF: 19 pg/mL
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Dynamic Range
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Depending on the desired application, the protocol can be applied to mouse or rat calibrators. Mouse EPO: 2.3-10000 pg/mL Rat EPO: 2.0-10000 pg/mL Mouse VEGF: 1.8-10000 pg/mL Rat VEGF: 19-10000 pg/mL
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Sample Type
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Serum, Plasma
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Recombinant standards
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Mouse EPO: Recombinant protein expressed in mouse myeloma cell line, NSO Rat EPO: Recombinant protein expressed in insect cell line Mouse VEGF: Recombinant protein expressed in insect cells Rat VEGF: Recombinant protein expressed in mouse myeloma cell line, NSO
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8 °C
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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Biomarkers of Tuberculosis Severity and Treatment Effect: A Directed Screen of 70 Host Markers in a Randomized Clinical Trial
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A hepcidin lowering agent mobilizes iron for incorporation into red blood cells in an adenine-induced kidney disease model of anemia in rats.
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Experimental pain and opioid analgesia in volunteers at high risk for obstructive sleep apnea
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Controlled regulation of erythropoietin by primary cultured renal cells for renal failure induced anemia.
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