The EGFR Signaling Family Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-EGFR, phospho-ErbB2, and phospho-IGF-1R in human whole cell lysates. The panel uses a single anti-phospho-typrosine detection antibody.
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Specifications
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Kit Contents
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References
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Resources
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Application(s)
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Phosphoproteins
,
Intracellular Signaling
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Analyte(s)
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EGFR, ErbB2, IGF-1R
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Species
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Human
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Instrument
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MESO QuickPlex SQ 120
,
SECTOR Imager 2400
,
SECTOR Imager 6000
,
SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Phospho-EGFR: Mouse Monoclonal
Phospho-ErbB2: Mouse Monoclonal
Phospho-IGF-1R: Mouse Monoclonal
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Detection Antibody
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Phospho-EGFR: Mouse Monoclonal
Phospho-ErbB2: Mouse Monoclonal
Phospho-IGF-1R: Mouse Monoclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8°C | 1 ea
(0.1 mL) |
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Phosphatase Inhibitor II 0.1 mL
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2-8°C | 1 ea
(1 ea) |
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Protease Inhibitor Solution 0.1 mL
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2-8°C | 1 ea
(1 ea) |
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8°C | 1 vial
(75 μL) |
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EGFR Family Plate
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2-8°C | 1 ea
(1 ea) |
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Tris Lysis Buffer
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2-8°C | 1 ea
(50 mL) |
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Tris Wash Buffer
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2-8°C | 1 ea
(200 mL) |
Read Buffer T
4X
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RT | 1 ea
(50 mL) |
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MSD Blocker A
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RT | 1 ea
(15 g) |
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MSD Blocker D-M, 0.2 mL
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≤-10°C | 1 ea
(1 ea) |
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MSD Blocker D-R, 0.05 mL
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≤-10°C | 1 ea
(1 ea) |
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8°C | 1 ea
(1 ea) |
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Phosphatase Inhibitor II 0.5mL
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2-8°C | 1 ea
(1 ea) |
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Protease Inhibitor Solution 0.5mL
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2-8°C | 1 ea
(1 ea) |
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8°C | 1 vial
(375 μL) |
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EGFR Family Plate
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2-8°C | 5 ea
(1 ea) |
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Tris Lysis Buffer
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2-8°C | 1 ea
(50 mL) |
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Tris Wash Buffer
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2-8°C | 1 ea
(200 mL) |
Read Buffer T
4X
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RT | 1 ea
(50 mL) |
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MSD Blocker A
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RT | 1 ea
(15 g) |
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MSD Blocker D-M, 0.9 mL
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≤-10°C | 1 ea
(1 ea) |
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MSD Blocker D-R, 0.2 mL
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≤-10°C | 1 ea
(1 ea) |
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8°C | 1 ea
(1 ea) |
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Phosphatase Inhibitor II 2.0mL
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2-8°C | 1 ea
(1 ea) |
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Protease Inhibitor Solution 2.0mL
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2-8°C | 1 ea
(1 ea) |
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SULFO-TAG Anti-Phospho-Tyrosine Antibody
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2-8°C | 4 vial
(375 μL) |
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EGFR Family Plate
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2-8°C | 20 ea
(1 ea) |
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Tris Lysis Buffer
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2-8°C | 1 ea
(200 mL) |
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Tris Wash Buffer
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2-8°C | 1 ea
(1000 mL) |
Read Buffer T
4X
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RT | 1 ea
(200 mL) |
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MSD Blocker A
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RT | 1 ea
(15 g) |
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MSD Blocker D-M, 0.9 mL
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≤-10°C | 4 ea
(1 ea) |
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MSD Blocker D-R, 0.2 mL
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≤-10°C | 4 ea
(1 ea) |
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Title
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Journal
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Year
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Targeting IGF-IR with ganitumab inhibits tumorigenesis and increases durability of response to androgen-deprivation therapy in VCaP prostate cancer xenografts.
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Dependence of Wilms tumor cells on signaling through insulin-like growth factor 1 in an orthotopic xenograft model targetable by specific receptor inhibition.
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Dual IGF-I/II-Neutralizing Antibody MEDI-573 Potently Inhibits IGF Signaling and Tumor Growth.
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Efficacy of Ganitumab (AMG 479), Alone and in Combination With Rapamycin, in Ewing's and Osteogenic Sarcoma Models.
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Chronic treatment with epoxyeicosatrienoic acids modulates insulin signaling and prevents insulin resistance in hepatocytes.
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View All
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Product Inserts
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SDS
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Datasheets
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Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters