Sign in to place orders and check the status of your recent orders.
Register NowRegistration allows you to enable online ordering, access productdocumentation, and review your online order history.
The Apoptosis Base Kit provides basic components for the quantitative determination of total p53, phospho-p53 (Ser15), PARP (cleaved), and Caspase-3 (cleaved) in human whole cell lysates. Caspase-3 is initially synthesized as a precursor protein but is activated by proteolytic cleavage of the N-terminus to produce the p20 and p17 cleavage (ASP175) products measured in this kit. PARP becomes inactive following cleavage (ASP214), however, the small cleavage product does retain its DNA-binding capabilities, blocking access to damaged DNA and inhibiting further DNA repair. Base kits generally include only MSD plates coated with the capture antibody, SULFO-TAG-labeled antibody, and MSD proprietary read buffer. (See product insert for a list of kit components). Base kits are most commonly used for high-throughput screening and assay development.