| FAQ | Category | |
Can I denature the cell lysates?
Yes, ionic detergents or urea can be used to denature proteins; however, these r... | Assays | |
Can I develop my own phosphoprotein assays?
Yes, immobilizing antibodies on bare plates and labeling detection antibodies is... | Assays | |
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Akt Kits?
Can I order additional calibrator for the Phospho-Akt (Ser473) Kits and Total Ak... | Assays | |
Can the assays be used with tissue lysates?
Yes, brain tissue homogenates, liver homogenates, homogenates from Matrigel plug... | Assays | |
How are MSD assays validated?
A cell model that exhibits regulation of a phosphoprotein of interest is selecte... | | |
How are your new Phospho-Akt (Ser473) and Total Akt Kits different than your existing kits?
Our new Phospho-Akt (Ser473) Kit (catalog# K150MND) and Total Akt Kit (catalog# ... | | |
How can I control cross-reactivity in multiplex assays?
MSD has several blockers available that can be added to lysis buffer or antibody... | Assays | |
How do I measure percent phosphorylation?
Perform a lysate titration to establish the linear range of the assay. For sampl... | Assays | |
Is a phospho-specific antibody always used to capture the phosphoprotein?
The phospho-specific antibody may be the capture or the detection antibody with ... | Assays | |
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Analysis of Results)
Why do you recommend normalization to total protein mass per well rather th... | Assays | |
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Product info)
Do the new total Akt and phospho-Akt (Ser473) assays use the same antibody... | Assays | |
FAQs for Phospho-Akt (Ser473) Kit and Total Akt Kits (Protocol clarification and modification)
1. I would like to run my samples for the Phospho-Akt (Ser473) Kits and Total Ak... | | |
Should I avoid any reagents in my lysis buffer?
High concentrations of ionic detergents (> 0.1%), urea (> 1 M), and other ... | | |
What amount (micrograms) of lysate should I use?
The performance of the assay will depend greatly on the abundance of the protein... | Assays | |
What other lysis buffers have been used with MSD assays?
Various modified RIPA buffers with low concentrations of ionic detergents and ur... | Assays
Reagents Diluents Buffers | |
What parameters can I optimize for my particular sample?
End users can vary the following parameters:
Concentration of detection a... | Assays | |
What standards are used to calibrate the assays?
The assays are developed using positive and negative control Whole Cell Lysate S... | Assays | |
What volume of lysate should I use?
A minimum of 25 µL should be placed in each well to ensure adequate covera... | Assays | |
When I add more lysate to increase assay signals, why do the signals continue to decrease?
High concentration of proteins in the sample can inhibit antibody binding, espec... | | |
Why do I not see the same dynamic range as I see with cytokine assays?
Non-specific binding of antibodies, especially phospho-specific antibodies, limi... | Assays | |
Will the new tau calibrator be incorporated into the other Tau assays?
MSD is currently working on new calibrators for our existing Total Tau and phosp... | Assays | |