Fast and sensitive detection of Bacillus anthracis spores by immunoassay.

Morel N, Volland H, Dano J, Lamourette P, Sylvestre P, Mock M, Créminon C.
Journal   Appl Environ Microbiol.
Analytes Measured  
Matrix Tested   Assay buffer
Year   2012
Page Numbers  
Application   Toxicology
Bacillus anthracis is one of the most dangerous potential biological weapons, and it is essential to develop a rapid and simple method to detect B. anthracis spores in environmental samples. Immunoassay is a rapid and easy-to-use method for the detection of B. anthracis by means of antibodies directed against surface spore antigens. With this objective in view, we have produced a panel of monoclonal antibodies against B. anthracis and developed colorimetric and electrochemiluminescence (ECL) immunoassays. Using the Meso Scale Discovery ECL technology(1), a detection limit ranging between 0.3 x 10(3) and 10(3) cfu/mL (i.e. 30 to 100 spores per test) was achieved depending on the B. anthracis strain. In complex matrices (5 mg/mL of soil or simulated powder), detection level (without any sample purification or concentration) was never altered more than three-fold compared with the results obtained in phosphate-buffered saline.

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