A validated assay to measure soluble IL-7 receptor shows minimal impact of IL-7 treatment.

Janot-Sardet, C., Assouline, B., Cheynier, R., Morre, M., Beq, S.
Journal   J Immunol Methods
Species  
Analytes Measured   IL-7
Matrix Tested   Human plasma, Rhesus plasma, Cynomolgus monkey plasma
Year   2010
Volume   353
Page Numbers   115-123
Application   Cytokines and Chemokines
Abstract
IL-7 is a crucial cytokine for T cell hematopoiesis and peripheral homeostasis which by signaling through its receptor alpha chain (CD127) is essential for inducing T cell proliferation and survival. Since the specific CD127 alpha chain is found in a soluble state (sCD127) and at a high level in plasma (ng/mL), it is important to develop a sensitive and reliable assay in order to investigate the potential role of this receptor in the regulation of IL-7 physiologic, physipathologic and therapeutic effects. We here report a fully validated method to measure sCD127 in human and simian plasma using a method based on ELISA MSD technology. We demonstrate that sCD127 is detectable at various levels in the plasma of healthy humans as well as in that of healthy Rhesus and Cynomolgus macaques (106.72, 205.26 and 366.95 ng/mL respectively). Moreover, as opposed to the sCD25/IL-2 tandem, we demonstrate that IL-7 treatment has no impact on sCD127 plasma concentration in patients infected by HIV.

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