PRAS40 (proline-rich Akt substrate, 40kDa), also known as Akt1s1, is a proline-rich substrate of Akt1. PRAS40 contains approximately 15% proline residues as opposed to normal proteins, which have about 5%. PRAS40 has a consensus site for Akt phosphorylation located at Thr246. In vitro experiments with purified Akt have shown phosphorylation of PRAS40 at Thr246. It has been demonstrated that there is decreased phosphorylation of PRAS40 at Thr246 in cells lacking Akt1 and Akt2. This site undergoes enhanced phosphorylation when PRAS40 is expressed in HEK293 cells that have constitutive expression of active Akt. It has been suggested that PRAS40 might be an inhibitor of kinase activity of mTORC1. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1.


PRAS40 Singleplex Products

Our PRAS40 immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of PRAS40. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.


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