NFkB

NFκB/p65 (nuclear factor kappa-light-chain-enhancer of activated B cells) refers to a family of five transcription factors which function downstream of receptors such as TNFR and the toll-like receptor superfamily. These transcription factors, which function as heterodimers and homodimers of the five NFκB family members, are involved in inflammation, immune response and development, proliferation, and cell survival. Inactive NFκB is normally found in the cytoplasm, bound by IκB (inhibitor of kappa B). Upon stimulation of an upstream receptor, IκB becomes phosphorylated and ubiquitinated and releases the NFκB which can translocate to the nucleus and function as a transcription factor. NFκB is activated by diverse stimuli such as reactive oxygen species, IL-1β, TNF, bacterial lipopolysaccharide, and ionizing radiation to name a few. NFκB stimulation is generally proliferative, proinflammatory, and anti-apoptotic and its upregulation in human cancers promotes tumor proliferation, metastasis, and chemoresistance. Unregulated NFκB activity also contributes to a number of inflammatory conditions, such as rheumatoid arthritis, Crohn’s disease, and ulcerative colitis. NFκB dysregulation has been seen in cancers of lymphoid origin, colitis associated cancers, hepatocellular carcinomas, and prostate cancer. Because of the relationship between NFκB and many diseased states, there has been much research into regulation of NFκB and the upstream members of this signal transduction cascade.

 

NFkB Singleplex Products

Our NFkB immunoassays provide fast, accurate, reproducible, and highly sensitive measurements of NFkB. MSD’s MULTI-ARRAY technology uses simple protocols, requires minimal sample, and quantifies analyte concentration over a wide dynamic range.

 

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Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

  • NFkB total - Human
  • Phospho-NFkB (Ser468) -
  • Phospho-NFkB (Ser536) -
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