BAD

Bcl-2-antagonist of cell death protein (BAD), a member of the pro-apoptotic Bcl-2 family of proteins, functions by displacing the binding of Bax to Bcl-2 and Bcl xL, and causes cell death by apoptosis. The binding of cytokines and growth factors to cell surface receptors activates intracellular signal transduction cascades that promote cell survival. Akt phosphorylates BAD on serine 136. BAD is also phosphorylated by protein kinase A (PKA) and p90 ribosomal S6 kinase (p90RSK) on serine 112. Phosphorylated BAD binds to members of the 14-3-3 protein family. This inhibits its interaction with Bcl-2 and Bcl-xL through cytosolic sequestration. The anti-apoptotic proteins Bcl-2 and BcL-xL are then free to interact with Apaf-1 and BID, thus promoting cell survival. MSD offers assay kits for quantitative determination of total and phosphorylated BAD at serine 112 in human and monkey whole cell lysates.

 

BAD Multiplex Products

Our multiplex panels let you process up to 10 analytes simultaneously in each well of a MULTI-SPOT microplate. They provide the same outstanding performance as our singleplex immmunoassays while saving money, sample, and time.

 

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Related Custom Assays

The following related assays are not in our catalog but may be available as custom orders. Custom orders are typically shipped in 3 to 6 weeks. You may request pricing and availability for the assays below using our Custom Assay Quote Request form.

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