MSD offers a range of individual assays utilizing U-PLEX Antibody Sets that provide a rapid and convenient method for measuring biomarkers in complex matrices. The individual assays are offered on MSD GOLD Small Spot Streptavidin Plates and use the same antibody sets and diluents as the U-PLEX multiplex assays. This allows for efficient transfer between the individual assay and a higher throughput multiplex configuration. Typical of assays developed on the MSD platform, the individual assays have high sensitivity, excellent precision, provide up to five-logs of linear dynamic range, and require minimal sample volume. Interferon gamma (IFN-γ), also known as immune interferon, is a glycosylated, 19.3 kDa pro-inflammatory cytokine. It exists as a non-covalently linked homodimer. IFN-γ dimers bind to the IFN-γ R1 (receptor 1), which is then triggered to bind the IFN-γ R2 (receptor 2) to form a functional receptor–ligand complex consisting of two receptor subunits. IFN-γ is produced by lymphocytes and is a potent activator of macrophages. It is involved in numerous pathways and is associated with a number of disorders including Huntington’s disease and hepatitis C. The U-PLEX NHP IFN-γ Assay provides the components for the quantitative determination of both natural and recombinant IFN-γ in Cynomolgus monkey and Rhesus monkey serum, plasma, and cell culture supernatants.

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