U-PLEX Mouse IL-17A/F Assay


U-PLEX Mouse IL-17A/F Assay Overview
The U-PLEX Mouse IL-17A/F Assay maximizes your flexibility to design an experiment that measures IL-17A/F alone or multiplexed with other MSD U-PLEX assays, your own antibody pairs, or a combination of both. This assay accurately quantitates native and recombinant mouse IL-17A/F in multiple sample types. To learn more about the advantages of U-PLEX assays click here.

U-PLEX Mouse IL-17A/F Assay Features
  • Measures mouse IL-17A/F in serum, plasma, and cell culture supernatants
  • Dynamic range: 0.61 pg/mL – 10,600 pg/mL
  • LLOD: 0.61 pg/mL

Typical Data
Calibration curve accuracy and precision for the U-PLEX Mouse IL-17A/F Assay were assessed and representative data are presented below.

Figure 1. U-PLEX Mouse IL-17A/F Calibration Curve
Mouse IL-17A/F Calibrator Curve
Figure 2. U-PLEX Mouse IL-17A/F Data
Mouse IL-17A/F Calibrator Curve Data
IL-17A/F Analyte

Description - Mouse interleukin 17A/F is a cross-linked heterodimer composed of IL-17A (CTLA8; Gene ID: 16171) and IL-17F (CANDF6, ML1; Gene ID: 257630) proteins that is secreted by IL-23 activated Th17 cells. IL-17A and IL-17F are both prototypical of the IL-17 family of cytokines. IL-17A/F binds to IL-17RA and recruits neutrophils with intermediate potency between IL-17A and IL-17F homodimers*.

Disease Focus/Application Area - Autoimmune and inflammatory diseases including asthma, rheumatoid arthritis, and inflammatory bowel disease (IBD).

*Note: IL-17A/F spot will capture but not detect I-17A. Do not combine Calibrator 7 (contains IL-17A) with Calibrator 12 (contains IL-17A/F) if measuring IL-17A/F.

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25 plates
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