NEW S-PLEX Mouse TNF-α Kit

S-PLEX Mouse TNF-a Calibrator Curve K152ADZS

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Individual S-PLEX Kits measure cytokines that are important in inflammatory response and immune system regulation. Built with S-PLEX technology, these assays are ultrasensitive, enabling you to reduce sample volume and conduct longitudinal studies with the same animal. The
S-PLEX Mouse TNF-α Assay has the essential components for an ultrasensitive assay that measures TNF-α in a single-analyte format.

Related Key Terms:
Tumor necrosis factor, TNF, tumor necrosis factor alpha, TNFα, cachexin, cachectin, TNF alpha, TNF-alpha, TNF-a, TNFalpha, TNFa, TNF a, TNF alpha, TNFα, TNF α, Tumor necrosis factor a, tumor necrosis factor ligand superfamily member 2, Tnfsf2, cachectin

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		TNF-α
Species		Mouse
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
LLOD (Sensitivity)		8.22 fg/mL
Dynamic Range		8.22 - 86,700 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant, CSF
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		21926
UniProt ID(s)		P06804
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K152ADZS-1

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	1 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	1 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 1 Plate	2-8 °C	1 vial (1 plate size)

S-PLEX Multiplex 96-Well 10-Spot SECTOR Plate	2-8 °C	1 each (1 each)

Diluent 100	2-8 °C	1 each (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

Diluent 68, 10 mL	2-8 °C	1 bottle (1 mL)

MSD GOLD Read Buffer B	RT	1 each (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 each (500 μL)

Blocker S2	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K152ADZS-2

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	5 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	5 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 5 Plate	2-8 °C	1 vial (5 plate size)

S-PLEX Multiplex 96-Well 10-Spot SECTOR Plate	2-8 °C	5 each (1 each)

Diluent 100	2-8 °C	1 each (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

Diluent 68, 50 mL	2-8 °C	1 bottle (1 mL)

MSD GOLD Read Buffer B	RT	1 each (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 each (500 μL)

Blocker S2	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K152ADZS-4

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	25 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	25 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 5 Plate	2-8 °C	5 vial (5 plate size)

S-PLEX Multiplex 96-Well 10-Spot SECTOR Plate	2-8 °C	25 each (1 each)

Diluent 100	2-8 °C	5 each (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

Diluent 68, 50 mL	2-8 °C	5 bottle (1 mL)

MSD GOLD Read Buffer B	RT	5 each (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 each (500 μL)

Blocker S2	≤-10 °C	5 vial (500 μL)

Kit Contents for Catalog No. K152ADZS-21

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	1 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	1 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 1 Plate	2-8 °C	1 vial (1 plate size)

S-PLEX Multiplex 96-Well 10-Spot QuickPlex Plate	2-8 °C	1 each (1 each)

Diluent 100	2-8 °C	1 each (50 mL)

Diluent 59	2-8 °C	1 bottle (8 mL)

Diluent 68, 10 mL	2-8 °C	1 bottle (1 mL)

MSD GOLD Read Buffer B	RT	1 each (18 mL)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

Blocker S1	≤-10 °C	1 each (500 μL)

Blocker S2	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K152ADZS-22

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	5 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	5 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 5 Plate	2-8 °C	1 vial (5 plate size)

S-PLEX Multiplex 96-Well 10-Spot QuickPlex Plate	2-8 °C	5 each (1 each)

Diluent 100	2-8 °C	1 each (50 mL)

Diluent 59	2-8 °C	1 bottle (40 mL)

Diluent 68, 50 mL	2-8 °C	1 bottle (1 mL)

MSD GOLD Read Buffer B	RT	1 each (90 mL)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

Blocker S1	≤-10 °C	1 each (500 μL)

Blocker S2	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K152ADZS-24

Product Description	Storage	Quantity per Kit
S-PLEX Proinflammatory Panel 1 (mouse) Calibrator Blend	≤-70 °C	25 vial (1 vial)

S-PLEX Proinflammatory Panel 1 (mouse) Coating Solution	≤-70 °C	25 vial (1 vial)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

TURBO-BOOST Mouse TNF-α Antibody, 5 Plate	2-8 °C	5 vial (5 plate size)

S-PLEX Multiplex 96-Well 10-Spot QuickPlex Plate	2-8 °C	25 each (1 each)

Diluent 100	2-8 °C	5 each (50 mL)

Diluent 59	2-8 °C	5 bottle (40 mL)

Diluent 68, 50 mL	2-8 °C	5 bottle (1 mL)

MSD GOLD Read Buffer B	RT	5 each (90 mL)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

Blocker S1	≤-10 °C	5 each (500 μL)

Blocker S2	≤-10 °C	5 vial (500 μL)

Title	Journal	Year
Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke Fiona Chapman, Sarah Jean Pour, Roman Wieczorek, Edgar Trelles Sticken, Jessica Budde, Karin Röwer, Sandra Otte, Elizabeth Mason, Lukasz Czekala, Thomas Nahde, Grant O’Connell, Liam Simms and Matthew Stevenson	Frontiers in Toxicology	2023
Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms Arvid Edén, Anna Grahn, Daniel Bremell, Anahit Aghvanyan, Pradeepthi Bathala, Dietmar Fuchs, Johanna Gostner, Lars Hagberg, Nelly Kanberg, Sunsanee Kanjananimmanont, Magnus Lindh, Salvia Misaghian, Staffan Nilsson, Michael Schöll, George Sigal, Erika Stentoft, Marie Studahl, Aylin Yilmaz, Mingyue Wang, Martin Stengelin, Henrik Zetterberg, Magnus Gisslén	JAMA Network Open	2022
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Anti-SARS-CoV-2 IgG from severely ill COVID-19 patients promotes macrophage hyper-inflammatory responses Willianne Hoepel, Hung-Jen Chen, Sona Allahverdiyeva, Xue Manz, Jurjan Aman, Amsterdam UMC COVID-19 Biobank, Peter Bonta, Philip Brouwer, Steven de Taeye, Tom Caniels, Karlijn van der Straten, Korneliusz Golebski, Guillermo Griffith, René Jonkers, Mads Larsen, Federica Linty, Annette Neele, Jan Nouta, Frank van Baarle, Cornelis van Drunen, Alexander Vlaar, Godelieve de Bree, Rogier Sanders, Lisa Willemsen, Manfred Wuhrer, Harm Jan Bogaard, Marit van Gils, Gestur Vidarsson, Menno de Winther, Jeroen den Dunnen	TBD	2020
Multisystem inflammatory syndrome in children and COVID-19 are distinct presentations of SARS–CoV-2 Caroline Diorio, Sarah E. Henrickson, Laura A. Vella, Kevin O. McNerney, Julie Chase, Chakkapong Burudpakdee, Jessica H. Lee, Cristina Jasen, Fran Balamuth, David M. Barrett, Brenda L. Banwell, Kathrin M. Bernt, Allison M. Blatz, Kathleen Chiotos, Brian T. Fisher, Julie C. Fitzgerald, Jeffrey S. Gerber, Kandace Gollomp, Christopher Gray, Stephan A. Grupp, Rebecca M. Harris, Todd J. Kilbaugh, Audrey R. Odom John, Michele Lambert, Emily J. Liebling, Michele E. Paessler, Whitney Petrosa, Charles Phillips, Anne F. Reilly, Neil D. Romberg, Alix Seif, Deborah A. Sesok-Pizzini, Kathleen E. Sullivan, Julie Vardaro, Edward M. Behrens, David T. Teachey, Hamid Bassiri	The Journal of Clinical Investigation	2020
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