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The Human ProInflammatory 7-Plex Tissue Culture Kit provides assay-specific components for the simultaneous quantitative determination of both natural and recombinant human IFN-γ, IL-1β, IL-6, IL-8, IL-10, IL-12p70, and TNF-α in tissue culture supernatant.
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Specifications
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Documentation
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References
Application(s)
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Cytokines & Chemokines
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Immunology/Inflammation
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Analyte(s)
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IFN-γ, IL-1β, IL-6, IL-8, IL-10, IL-12p70, TNF-α
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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IFN-γ: Mouse Monoclonal IL-1β: Mouse Monoclonal IL-6: Mouse Monoclonal IL-8: Mouse Monoclonal IL-10: Rat Monoclonal IL-12p70: Rat Monoclonal TNF-α: Mouse Monoclonal
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Detection Antibody
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IFN-γ: Mouse Monoclonal IL-1β: Goat Polyclonal IL-6: Goat Polyclonal IL-8: Goat Polyclonal IL-10: Rat Monoclonal IL-12p70: Mouse Monoclonal TNF-α: Goat Polyclonal
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LLOD (Sensitivity)
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IFN-γ: 3.2 pg/mL IL-1β: 0.57 pg/mL IL-6: 0.13 pg/mL IL-8: 0.15 pg/mL IL-10: 0.32 pg/mL IL-12p70: 0.23 pg/mL TNF-α: 0.69 pg/mL
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Dynamic Range
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IFN-γ: 3.2-10000 pg/mL IL-1β: 0.57-10000 pg/mL IL-6: 0.13-10000 pg/mL IL-8: 0.15-10000 pg/mL IL-10: 0.32-10000 pg/mL IL-12p70: 0.23-10000 pg/mL TNF-α: 0.69-10000 pg/mL
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Recombinant standards
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IFN-γ, IL-1β, IL-6, IL-8, TNF-α: Recombinant protein expressed in E. coli IL-10, IL-12p70: Expressed in insect cells
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Title
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Journal
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Year
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Twenty-eight day repeated exposure of human 3D bronchial epithelial model to heated tobacco aerosols indicates decreased toxicological responses compared to cigarette smoke
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Viral Antigen and Inflammatory Biomarkers in Cerebrospinal Fluid in Patients With COVID-19 Infection and Neurologic Symptoms Compared With Control Participants Without Infection or Neurologic Symptoms
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Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations
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Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection
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IL-6 as a Mediator of the Association Between Traditional Risk Factors and Future Myocardial Infarction: A Nested Case-Control Study
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