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Human Bone Panel I Kit

The Human Bone Marker Panel I Kit provides assay-specific components for the quantitative determination of both natural and recombinant human osteoprotegerin (OPGN), sclerostin (SOST), and alkaline phosphatase (ALP) in serum and plasma.

Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.

Specifications
Kit Contents
References
Documentation

Application(s)		Bone Disorders
Analyte(s)		ALP, Osteoprotegerin, Sclerostin
Species		Human
Instrument		MESO QuickPlex SQ 120 , SECTOR Imager 2400 , SECTOR Imager 6000 , MESO SECTOR S 600
Plate Type		96-well
Capture Antibody		Osteoprotegerin: Goat Polyclonal Sclerostin: Goat Polyclonal Alkaline Phosphatase: Mouse Monoclonal
Detection Antibody		Osteoprotegerin: Mouse Monoclonal Sclerostin: Goat Polyclonal Alkaline Phosphatase: Mouse Monoclonal
Dynamic Range		Osteoprotegerin: 0.004 - 200 ng/mL Sclerostin: 0.004 - 100 ng/mL Alkaline Phosphatase: 1.9 - 4000 ng/mL
Sample Type		Serum, Plasma
Recombinant standards		Osteoprotegerin: Recombinant human protein expressed in a mouse myeloma cell line Sclerostin: Recombinant human protein expressed in a mouse myeloma cell line Alkaline Phosphatase: Recombinant human protein expressed in a mouse myeloma cell line
LLOD (Sensitivity)		Osteoprotegerin: 0.004 ng/mL Sclerostin: 0.004 ng/mL Alkaline Phosphatase: 1.9 ng/mL
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Statement(s)		Please refer to the product insert for the storage conditions of individual kit components.
Storage Condition		Multi-Component

Kit Contents for Catalog No. K15146C-1

Product Description	Storage	Quantity per Kit
Human Bone Panel I Calibrator	≤-70°C	1 vial (150 μL)

SULFO-TAG Anti-human ALP Antibody	2-8°C	1 vial (75 μL)

SULFO-TAG Anti-human Osteoprotegerin Antibody	2-8°C	1 vial (75 μL)

SULFO-TAG Anti-human Sclerostin Antibody	2-8°C	1 vial (75 μL)

Human Bone Panel I Plate	2-8°C	1 ea (1 ea)

Diluent 100	2-8°C	1 ea (50 mL)

Diluent 7	≤-10°C	1 ea (5 mL)

Diluent 11	≤-10°C	1 ea (5 mL)

Read Buffer T 4X	RT	1 ea (50 mL)

MSD Blocker A	RT	1 ea (250 mL)

MSD Phosphate Buffer 5X	RT	1 ea (50 mL)

Kit Contents for Catalog No. K15146C-2

Product Description	Storage	Quantity per Kit
Human Bone Panel I Calibrator	≤-70°C	5 vial (150 μL)

SULFO-TAG Anti-human ALP Antibody	2-8°C	1 vial (375 μL)

SULFO-TAG Anti-human Osteoprotegerin Antibody	2-8°C	1 vial (375 μL)

SULFO-TAG Anti-human Sclerostin Antibody	2-8°C	1 vial (375 μL)

Human Bone Panel I Plate	2-8°C	5 ea (1 ea)

Diluent 100	2-8°C	1 ea (50 mL)

Diluent 7	≤-10°C	1 ea (50 mL)

Diluent 11	≤-10°C	1 ea (50 mL)

Read Buffer T 4X	RT	1 ea (50 mL)

MSD Blocker A	RT	1 ea (250 mL)

MSD Phosphate Buffer 5X	RT	1 ea (50 mL)

Kit Contents for Catalog No. K15146C-3

Product Description	Storage	Quantity per Kit
Human Bone Panel I Calibrator	≤-70°C	20 vial (150 μL)

SULFO-TAG Anti-human ALP Antibody	2-8°C	4 vial (375 μL)

SULFO-TAG Anti-human Osteoprotegerin Antibody	2-8°C	4 vial (375 μL)

SULFO-TAG Anti-human Sclerostin Antibody	2-8°C	4 vial (375 μL)

Human Bone Panel I Plate	2-8°C	20 ea (1 ea)

Diluent 100	2-8°C	2 ea (50 mL)

Diluent 7	≤-10°C	2 ea (50 mL)

Diluent 11	≤-10°C	2 ea (50 mL)

Read Buffer T 4X	RT	1 ea (200 mL)

MSD Blocker A	RT	1 ea (1 l)

MSD Phosphate Buffer 5X	RT	1 ea (200 mL)

Order Details

Catalog No

Size

Price in USD

Qty

K15146C-2

5 plates

$5,720.00

Made to order (25 plates minimum); 6-8 week lead time

Contact Information

Customer Service/Orders
1-240-314-2795

5:00 a.m. to 8:00 p.m.
US Eastern Time

Email

Scientific/Technical Support
1-240-314-2798

8:30 a.m. to 5:30 p.m.
US Eastern Time

Email

Instrument Support
1-301-947-2057

8:30 a.m. - 5:30 p.m.
US Eastern Time

Email

Company Headquarters
1-240-314-2600
1601 Research Blvd.
Rockville, Maryland 20850-3173
USA

Title	Journal	Year
Development of a multi-biomarker disease activity test for rheumatoid arthritis. Centola M, Cavet G, Shen Y, Ramanujan S, Knowlton N, Swan KA, Turner M, Sutton C, Smith DR, Haney DJ, Chernoff D, Hesterberg LK, Carulli JP, Taylor PC, Shadick NA, Weinblatt ME, Curtis JR.	PLoS One	2013
First missense mutation in the SOST gene causing sclerosteosis by loss of sclerostin function. Piters E, Culha C, Moester M, Van Bezooijen R, Adriaensen D, Mueller T, Weidauer S, Jennes K, de Freitas F, Lowik C, Timmermans JP, Van Hul W, Papapoulos S.	Hum Mutat.	2010
Serum correlates of the placebo effect in irritable bowel syndrome. Kokkotou, E., Conboy, L.A., Ziogas, D.C., Quilty, M.T., Kelley, J.M., Davis, R.B., Lembo, A.J., Kaptchuk, T.J.	Neurogastroenterol Motil.	2010
Sex and ethnic differences in 47 candidate proteomic markers of cardiovascular disease: the mayo clinic proteomic markers of arteriosclerosis study. Kim, C.X., Bailey, K.R., Klee, G.G., Ellington, A.A., Liu, G., Mosley, T.H. Jr, Rehman, H., Kullo, I.J.	Cancer Chemother Pharmacol.	2010

Product Inserts		Human Bone Panel I
SDS		Blocker A - English Diluent 10, Diluent 11, Diluent 12, Diluent 13, Diluent 15, Diluent 16 and Diluent 17 - English Diluent 100 and Diluent 101 - English Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 - English MSD Calibrators and Controls - English Phosphate Buffer (5x) - English Read Buffer T (4x), Read Buffer S (4x) and Read Buffer S (1x) - English
Datasheets		DS Human bone Panel I

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Human Bone Panel I Kit

Kit Contents for Catalog No. K15146C-1

Kit Contents for Catalog No. K15146C-2

Kit Contents for Catalog No. K15146C-3

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