U-PLEX NHP IL-17A/F Assay Overview
The U-PLEX NHP IL-17A/F Assay maximizes your flexibility to design an experiment that measures IL-17A/F alone or multiplexed with other MSD U-PLEX assays, your own antibody pairs, or a combination of both. To learn more about the advantages of U-PLEX assays click here.

U-PLEX NHP IL-17A/F Assay Features
  • Measures NHP IL-17A/F in serum, plasma, and cell culture supernatants
  • Dynamic range: 1.8 pg/mL – 18,400 pg/mL
  • LLOD: 1.8 pg/mL

Typical Data
Calibration curve accuracy and precision for the U-PLEX NHP IL-17A/F assay were assessed and representative data are presented below.

Figure 1. U-PLEX NHP IL-17A/F Calibration Curve
NHP IL-17A/F Calibrator Curve
Figure 2. U-PLEX NHP IL-17A/F Data
NHP IL-17A/F Calibrator Curve Data
IL-17A/F Analyte

Description - NHP interleukin 17A/F is a cross-linked heterodimer composed of IL-17A (IL-17, CTLA8; Gene ID: 708123) and IL-17F (CANDF6, ML1; Gene ID: 708220) proteins that is secreted by IL-23 activated Th17 cells. IL-17A and IL-17F are both prototypical of the IL-17 family of cytokines. IL-17A/F binds to IL-17RA and recruits neutrophils with intermediate potency between IL-17A and IL-17F homodimers*.

Disease Focus/Application Area - Autoimmune and inflammatory diseases including asthma, rheumatoid arthritis, and inflammatory bowel disease (IBD).

*Note: IL-17A/F spot will capture but not detect I-17A. Do not combine Calibrator 6 (contains IL-17A/F and IL-17F) with Calibrator 1 (contains IL-17A) if measuring IL-17A/F.

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