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S-PLEX Human IL-4 Kit

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S-PLEX is MSD’s ultra-sensitive assay platform. It can dramatically improve the sensitivity of immunoassays, reducing the lower limit of detection (LLOD) by 10- to 1000-fold over other assay methods. The S-PLEX Human IL-4 Assay has the essential components for an ultrasensitive assay that measures IL-4 in a single-analyte format. For detailed instructions on running the IL-4 assay, please see the product insert in the "Documentation" tab below.

Related Key Terms:
IL4, BCGF-1, BCGF1, BSF-1, BSF1, interleukin 4, intrinsic asthma, vernal conjunctivitis

Specifications
Kit Contents
Documentation
References

Application(s)		Cytokines & Chemokines , Immunology/Inflammation
Analyte(s)		IL-4
Species		Human
Plate Type		96-well
Instrument(s)		MESO SECTOR S 600MM , MESO QuickPlex SQ 120MM , MESO QuickPlex Q 60MM , MESO SECTOR S 600 , MESO QuickPlex SQ 120 , SECTOR Imager 6000 , SECTOR Imager 2400
Capture Antibody		Mouse monoclonal
Detection Antibody		Mouse monoclonal
LLOD (Sensitivity)		0.54 fg/mL
Dynamic Range		0.54 - 9,000 fg/mL
Sample Type		Serum, Plasma, Cell Culture Supernatant
Recombinant standards		Expressed in E. coli
Usage Statement		For Research Use Only. Not for use in diagnostic procedures.
Storage Condition		Multi-Component
Gene ID(s)		3565
UniProt ID(s)		P05112
Schedule B Code		3822.19.0000

Kit Contents for Catalog No. K151A3S-1

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-4 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 21 (includes Diluent 2 – 8 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151A3S-2

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-4 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151A3S-4

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-4 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2 (200X)	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1 (4X)	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well SECTOR Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1 (4X)	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3 (200X)	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Kit Contents for Catalog No. K151A3S-21

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	1 each (1 plate size)

Human IL-4 Calibrator	2-8 °C	1 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	1 each (50 μL)

S-PLEX Detect D1	≤-70 °C	1 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	1 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 21 (includes Diluent 2 – 8 mL, and Diluent 3 – 8 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1	≤-10 °C	1 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	1 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	1 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (18 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151A3S-22

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	1 each (5 plate size)

Human IL-4 Calibrator	2-8 °C	5 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	1 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	5 each (50 μL)

S-PLEX Detect D1	≤-70 °C	5 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	5 each (1 plate)

Diluent 100	2-8 °C	1 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	1 each (1 each)

S-PLEX Enhance E1	≤-10 °C	5 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	5 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	5 each (50 μL)

MSD GOLD Read Buffer A	RT	1 bottle (90 mL)

Blocker S1	≤-10 °C	1 vial (500 μL)

Kit Contents for Catalog No. K151A3S-24

Product Description	Storage	Quantity per Kit
S-PLEX Human IL-4 Antibody Set	2-8 °C	5 each (5 plate size)

Human IL-4 Calibrator	2-8 °C	25 vial (1 vial)

S-PLEX Coating Reagent C1	≤-70 °C	5 vial (300 μL)

S-PLEX Detect D2	≤-70 °C	25 each (50 μL)

S-PLEX Detect D1	≤-70 °C	25 each (1.7 mL)

S-PLEX 96-Well QuickPlex Plate	2-8 °C	25 each (1 plate)

Diluent 100	2-8 °C	5 bottle (50 mL)

Diluent Assembly 22 (includes Diluent 2 – 40 mL, and Diluent 3 – 40 mL)	≤-10 °C	5 each (1 each)

S-PLEX Enhance E1	≤-10 °C	25 each (1.7 mL)

S-PLEX Enhance E2	≤-10 °C	25 vial (1.7 mL)

S-PLEX Enhance E3	≤-70 °C	25 each (50 μL)

MSD GOLD Read Buffer A	RT	5 bottle (90 mL)

Blocker S1	≤-10 °C	5 vial (500 μL)

Order Details

K151A3S

Select your instrument:

S 600

SQ 120

Q 60

Catalog No

Size

Price in USD

Qty

K151A3S-1

1 plate size

$882.00

Usually ships in 1-3 days

K151A3S-2

5 plate size

$3,735.00

Usually ships in 1-3 days

K151A3S-4

25 plate size

$15,873.00

Usually ships in 1-3 days

K151A3S-21

1 each

$882.00

Usually ships in 1-3 days

K151A3S-22

5 each

$3,735.00

Usually ships in 1-3 days

K151A3S-24

25 each

$15,873.00

Usually ships in 1-3 days

Contact Information

Meso Scale Japan 株式会社
電話: (050) 1706-7074
〒103-0027
東京都中央区日本橋三丁目９番１号日本橋三丁目スクエア 11階 Vistra　Japan内

Product Inserts		S-PLEX Human IL-4 Kit Product Insert
Certificate of analysis		K00S0165 K00S0140 K00S0094 K00S0052 with Extension K00S0025 K00S0041 K00S0002A
SDS		Biotin-conjugated Antibodies Safety Data Sheet Diluent 2, Diluent 3, Diluent 5, Diluent 7 and Diluent 8 Diluent 50, Diluent 56, Diluent 61, Diluent 100, and Diluent 101 MSD GOLD Read Buffer A

Title	Journal	Year
Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Bing Sun, Norina Tang, Michael J. Peluso, Nikita S. Iyer, Leonel Torres, Joanna L. Donatelli, Sadie E. Munter, Christopher C. Nixon, Rachel L. Rutishauser, Isabel Rodriguez-Barraquer, Bryan Greenhouse, John D. Kelly, Jeffrey N. Martin, Steven G. Deeks, Timothy J. Henrich, and Lynn Pulliam	Cells	2021
Galectin-9, a Player in Cytokine Release Syndrome and a Surrogate Diagnostic Biomarker in SARS-CoV-2 Infection Najmeh Bozorgmehr, Siavash Mashhouri, Eliana Perez Rosero, Lai Xu, Shima Shahbaz, Wendy Sligl, Mohammed Osman, Demetrios J. Kutsogiannis, Erika MacIntyre, Conar R. O’Neil, Shokrollah Elahi	mBio	2021
Reliability of self-sampling for accurate assessment of respiratory virus viral and immunologic kinetics Alpana Waghmare, Elizabeth M Krantz, Subhasish Baral, Emma Vasquez, Tillie Loeffelholz, E. Lisa Chung, Urvashi Pandey, Jane Kuypers, Elizabeth R Duke, Keith R Jerome, Alexander L Greninger, Daniel B Reeves, Florian Hladik, E Fabian Cardozo-Ojeda, Michael Boeckh, Joshua T Schiffer	TBD	2020
Early and late effects of maternal experience on hippocampal neurogenesis, microglia, and the circulating cytokine milieu Rand S. Eid, Jessica A. Chaiton, Stephanie E. Lieblich, Tamara S. Bodnar, Joanne Weinberg, Liisa A.M. Galea	Neurobiology of Aging	2019
Cytokine biomarkers in tear film for primary open-angle glaucoma Gupta D, Wen JC, Huebner JL, Stinnett S, Kraus VB, Tseng HC, Walsh M.	Clin Ophthalmol.	2017
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S-PLEX Human IL-4 Kit

Kit Contents for Catalog No. K151A3S-1

Kit Contents for Catalog No. K151A3S-2

Kit Contents for Catalog No. K151A3S-4

Kit Contents for Catalog No. K151A3S-21

Kit Contents for Catalog No. K151A3S-22

Kit Contents for Catalog No. K151A3S-24

Order Details

K151A3S

S 600

SQ 120

Q 60

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