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The Phospho(Ser473)/Total Akt Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-Akt (Ser473) and total Akt in human, mouse, and rat whole cell lysates.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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Akt
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
,
MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
,
MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Phospho-Akt: Mouse Monoclonal
Total Akt: Mouse Monoclonal
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Detection Antibody
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Phospho-Akt: Mouse Monoclonal
Total Akt: Mouse Monoclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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2-8 °C
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Schedule B Code
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3002.15.0100
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8 °C
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1 each
(0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Akt Antibody
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2-8 °C
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1 vial
(75 μL)
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Phospho(Ser473)/Total Akt Plate
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2-8 °C
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1 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Akt Antibody
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2-8 °C
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1 vial
(375 μL)
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Phospho(Ser473)/Total Akt Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 2.0mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Akt Antibody
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2-8 °C
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4 vial
(375 μL)
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Phospho(Ser473)/Total Akt Plate
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2-8 °C
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20 each
(1 each)
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2-8 °C
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1 bottle
(200 mL)
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2-8 °C
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1 bottle
(1000 mL)
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RT
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1 bottle
(200 mL)
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RT
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1 bottle
(15 g)
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Product Inserts
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Datasheets
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Certificate of analysis
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SDS
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Title
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Journal
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Year
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Discovery of diverse and functional antibodies from large human repertoire antibody libraries.
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Dual blockade of the PI3K/AKT/mTOR (AZD8055) and RAS/MEK/ERK (AZD6244) pathways synergistically inhibits rhabdomyosarcoma cell growth in vitro and in vivo.
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Functional Consequences of the Macrophage Stimulating Protein 689C Inflammatory Bowel Disease Risk Allele.
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Multimodal microvascular imaging reveals that selective inhibition of class I PI3K is sufficient to induce an antivascular response.
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S49076 is a novel kinase inhibitor of MET, AXL and FGFR with strong preclinical activity alone and in association with bevacizumab.
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