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The transcription factor p53 can be phosphorylated at Ser15 by a number of kinases including ATM, ATR, and DNAPK. This phosphorylation event results in the accumulation of p53 protein by preventing the binding of the negative regulatory protein MDM2 and eliminating its targeted degradation. Activated p53 induces the transcription of many genes, including those affecting cell cycle progression. This kit provides assay-specific components for the quantitative determination of phospho-p53 (Ser15) in human whole cell lysates.
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Specifications
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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p53
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Species
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Human
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Instrument(s)
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SECTOR Imager 2400
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SECTOR Imager 6000
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MESO QuickPlex SQ 120
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MESO QuickPlex SQ 120MM
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MESO SECTOR S 600
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MESO SECTOR S 600MM
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Plate Type
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96-well
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Capture Antibody
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Mouse Monoclonal
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Detection Antibody
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Goat Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
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Identification of inhibitors for MDM2 ubiquitin ligase activity from natural product extracts by a novel high-throughput electrochemiluminescent screen.
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Multiplexing siRNAs to compress RNAi-based screen size in human cells.
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Customer Service/Orders
Scientific/Technical Support
Instrument Support
Company Headquarters