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Histone H3 is one of the five main classes of histones, which are found in the nucleus of eukaryotic cells, and are the main building blocks of chromatin. It has an amino terminal tail and post-translational modification of this tail is involved in chromatin relaxation and transcriptional regulation. Such epigenetic modifications of histones have been shown to play a role in cancer, embryonic stem cell function, and mammalian development. This kit provides assay-specific components for the quantitative determination of phospho-Histone H3 (Ser10) in human, mouse, and rat whole cell lysates.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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Histone H3
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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Rabbit Polyclonal
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Detection Antibody
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Rabbit Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3002.15.0100
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.1 mL
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2-8 °C
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1 each
(0.1 mL)
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Phosphatase Inhibitor II 0.1 mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.1 mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Phospho-Histone H3 (Ser10) Antibody
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2-8 °C
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1 vial
(75 μL)
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Histone H3 Plate
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2-8 °C
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1 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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MSD Blocker D-R, 0.05 mL
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≤-10 °C
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1 vial
(1 vial)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Phospho-Histone H3 (Ser10) Antibody
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2-8 °C
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1 vial
(375 μL)
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Histone H3 Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 2.0mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 2.0mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 2.0mL
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2-8 °C
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1 each
(1 each)
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SULFO-TAG Anti-Phospho-Histone H3 (Ser10) Antibody
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2-8 °C
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4 vial
(375 μL)
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Histone H3 Plate
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2-8 °C
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20 each
(1 each)
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2-8 °C
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1 bottle
(200 mL)
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2-8 °C
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1 bottle
(1000 mL)
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RT
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1 bottle
(1000 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(1 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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A phase I pharmacokinetic and pharmacodynamic study of CHR-3996, an oral class I selective histone deacetylase inhibitor in refractory solid tumors.
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Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activity.
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