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The ERK-STAT3 Signaling Cascade Whole Cell Lysate Kit provides assay-specific components for the quantitative determination of phospho-MEK1/2 (Ser217/221), phospho-ERK-1/2 (Thr/Tyr: 202/204; 185/187), and phospho-STAT3 (Tyr705) in human, mouse, and rat whole cell lysates.
Please note: MSD has transitioned this product to a status of “made-to-order” with a minimum order size of 25 plates. For an order of the 5-plate pack size, the minimum quantity is 5 packs (25 plates). The 1-plate and 20-plate pack sizes have been discontinued.
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Specifications
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Kit Contents
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Documentation
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References
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Application(s)
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Phosphoproteins
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Intracellular Signaling
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Analyte(s)
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ERK-1/2 (total), MEK 1/2, STAT3
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Species
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Human, Mouse, Rat
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Instrument(s)
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SECTOR Imager 2400
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MESO SECTOR S 600MM
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SECTOR Imager 6000
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MESO QuickPlex SQ 120MM
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MESO QuickPlex SQ 120
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MESO SECTOR S 600
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Plate Type
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96-well
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Capture Antibody
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ERK1/2: Mouse Monoclonal
MEK1/2: Mouse Monoclonal
STAT3: Mouse Monoclonal
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Detection Antibody
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ERK1/2: Mouse Monoclonal
MEK1/2: Rabbit Polyclonal
STAT3: Rabbit Polyclonal
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Usage Statement
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For Research Use Only. Not for use in diagnostic procedures.
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Kit Model
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Whole Cell Lysate
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Storage Statement(s)
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Please refer to the product insert for the storage conditions of individual kit components.
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Storage Condition
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Multi-Component
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Schedule B Code
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3002.15.0100
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Product Description
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Storage
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Quantity per Kit
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Phosphatase Inhibitor I 0.5mL
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2-8 °C
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1 each
(1 each)
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Phosphatase Inhibitor II 0.5mL
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2-8 °C
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1 each
(1 each)
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Protease Inhibitor Solution 0.5mL
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2-8 °C
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1 each
(1 each)
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PMSF in DMSO, 0.250 mL
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≤-10 °C
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1 each
(1 each)
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SULFO-TAG Anti-Total Erk1/2 Antibody
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2-8 °C
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1 vial
(375 μL)
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SULFO-TAG Anti-Phospho-MEK1/2 Antibody
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2-8 °C
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1 vial
(500 μL)
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SULFO-TAG Anti-Phospho-STAT3 Antibody
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2-8 °C
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1 vial
(375 μL)
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ERK-STAT3 Cascade Plate
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2-8 °C
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5 each
(1 each)
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2-8 °C
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1 bottle
(50 mL)
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2-8 °C
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1 bottle
(200 mL)
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RT
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1 bottle
(50 mL)
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RT
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1 bottle
(15 g)
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≤-10 °C
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1 vial
(0.2 mL)
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Product Inserts
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Datasheets
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SDS
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Title
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Journal
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Year
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A randomized, placebo-controlled study of the pharmacokinetics, pharmacodynamics, and tolerability of the oral JAK2 inhibitor fedratinib (SAR302503) in healthy volunteers.
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Characterization of a high-affinity human antibody with a disulfide bridge in the third complementarity-determining region of the heavy chain.
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Mechanism of anti-glioma activity and in vivo efficacy of the cannabinoid ligand KM-233.
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Development and Utilization of Activated STAT3 Detection Assays for Screening a Library of Secreted Proteins.
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Cooperative signaling through the signal transducer and activator of transcription 3 and nuclear factor-{kappa}B pathways in subtypes of diffuse large B-cell lymphoma.
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Order Details
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Size
Price in USD
Qty
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Scientific/Technical Support
Instrument Support
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